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Sci Rep

Title:		Profiles of volatile indole emitted by Escherichia coli based on CDI-MS
Author(s):		Zhong Q; Cheng F; Liang J; Wang X; Chen Y; Fang X; Hu L; Hang Y;
Address:		"Department of clinical laboratory, Jiangxi Province Key Laboratory of Laboratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, P.R. China. Department of clinical laboratory, Jiangxi Chest (third people) Hospital, Nanchang, 330006, P.R. China. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, 330013, P.R. China. Department of clinical laboratory, Jiangxi Province Key Laboratory of Laboratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, P.R. China. longhuahu@163.com. Department of clinical laboratory, Jiangxi Province Key Laboratory of Laboratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, P.R. China. bingbinghang@163.com"
Journal Title:		Sci Rep
Year:		2019
Volume:		20190911
Issue:		1
Page Number:		13139 -
DOI:		10.1038/s41598-019-49436-y
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Escherichia coli is an important pathogen of nosocomial infection in clinical research, Thus, exploring new methods for the rapid detection of this pathogen is urgent. We reported the early release of molecular volatile indole vapour of E. coli cultures and blood cultures analyzed by direct atmospheric corona discharge ionization mass spectrometry (CDI-MS). The concentration of indole in E. coli cultures remarkably increases during the early log and lag phases of bacterial growth, thereby enabling early detection. Technical replicates were cultivated for 3 days for reference diagnosis using current conventional bacteraemia detection. A reference MS screen of common microbes from other genera confirmed that the peaks at m/z 116 signal corresponded to indole were specifically present in E. coli. Our results indicated that volatile indole based on CDI-MS without the need for any sample pretreatment is highly suitable for the reliable and cost-efficient differentiation of E. coli, especially for bacteraemia in humans"
Keywords:		"Bacteremia/microbiology Cross Infection/microbiology Escherichia coli/*metabolism/physiology Escherichia coli Infections/microbiology Humans Indoles/*analysis Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*meth;"
Notes:		"MedlineZhong, Qiaoshi Cheng, Feng Liang, Juchao Wang, Xiaozhong Chen, Yanhui Fang, Xueyao Hu, Longhua Hang, Yaping eng Research Support, Non-U.S. Gov't England 2019/09/13 Sci Rep. 2019 Sep 11; 9(1):13139. doi: 10.1038/s41598-019-49436-y"