| Title: | Recovery from reproductive and morphological abnormalities in medaka (Oryzias latipes) following a 14-day exposure to diclofenac |
| Author(s): | Yokota H; Higashi K; Hanada E; Matsuzaki E; Tsuruda Y; Suzuki T; Nakano E; Eguchi S; |
| Address: | "Department of Biosphere Sciences, School of Human Sciences, Kobe College, Nishinomiya-shi, Hyogo, Japan. Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, Japan" |
| ISSN/ISBN: | 1552-8618 (Electronic) 0730-7268 (Linking) |
| Abstract: | "Mating pairs of medaka (Oryzias latipes) were exposed to diclofenac at measured concentrations of 0 (control), 7.1, 37, and 78 mug/L for 14 d under static-renewal conditions. Effects on reproductive success, as well as morphological abnormalities, of the fish were assessed. During the exposure period, both fecundity and fertility were significantly decreased in the 37- and 78-mug/L treatment groups, and swollen abdomens in females were observed in all exposure groups. Notably, a defect of the lower jaw was also observed in 4 male fish: 2 at 37 mug/L and 2 at 78 mug/L of diclofenac. Subsequently, we investigated whether the reproductive and morphological abnormalities induced by diclofenac would be permanent or reversible once the medaka were returned to clean water. The reproductive ability of paired medaka was gradually restored to fish that were cultured in clean water for 14 d. After this period in clean water, we also observed a noticeable decrease in swollen abdomens in females; however, mandibular defects in the males remained, even after the 14-d recovery period. Radiographic and histochemical examinations revealed that diclofenac might affect bone remodeling in the lower jaw of male medaka because of a disruption in osteoclast function. These results suggest that reproductive impairments in pairs of medaka exposed to diclofenac may be reversible but that skeletal deformities (i.e., mandibular defect) in males may be persistent. Environ Toxicol Chem 2017;36:3277-3283. (c) 2017 SETAC" |
| Keywords: | "Animals Anti-Inflammatory Agents, Non-Steroidal/*toxicity Bone Remodeling/drug effects Diclofenac/*toxicity Female Fertility/drug effects Male Oryzias/abnormalities/*physiology Reproduction/drug effects Water Pollutants, Chemical/*toxicity Fish Mandibular;" |
| Notes: | "MedlineYokota, Hirofumi Higashi, Kaho Hanada, Erina Matsuzaki, Ei Tsuruda, Yukinari Suzuki, Tomoko Nakano, Eiko Eguchi, Sayaka eng 2017/06/22 Environ Toxicol Chem. 2017 Dec; 36(12):3277-3283. doi: 10.1002/etc.3899. Epub 2017 Sep 14" |
