| Title: | "Two putative pheromone receptors, but not their cognate pheromones, regulate female fertility in the atypical mating fungus Colletotrichum fructicola" |
| Author(s): | Yao L; Kong Y; Yang W; Tian H; Meng X; Zhao X; Zhang R; Sun G; Rollins JA; Liang X; |
| Address: | "Northwest A&F University, 12469, Yangling, Shaanxi, China; 2817455904@qq.com. Northwest A&F University, 12469, Yangling, Shaanxi, China; 1443908108@qq.com. Yangling, China; 2954080798@qq.com. Yangling, China; 1329945151@qq.com. Yangling, China; 2457068226@qq.com. Northwest A&F University, 12469, Yangling, Shaanxi, China; 3192332967@qq.com. Yangling, Shannxi, China; rongzh@nwsuaf.edu.cn. College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, China, Yangling, Shannxi, China, 712100; sgy@nwsuaf.edu.cn. Univ of Florida, PO Box 110680 - Plant Path Dept, 1453 Fifield Hall, Gainesville, Florida, United States, 32611-0680; rollinsj@ufl.edu. 308 Plant protection collegenorthwest a&F universityyangling, shaanxi, China, 712100; xiaofeiliang@nwsuaf.edu.cn" |
| DOI: | 10.1094/PHYTO-11-22-0436-R |
| ISSN/ISBN: | 0031-949X (Print) 0031-949X (Linking) |
| Abstract: | "Colletotrichum fungi are a group of damaging phytopathogens with atypical mating type loci (harboring only MAT1-2-1 but not MAT1-1-1) and complex sexual behaviors. Sex pheromones and their cognate G-protein coupled receptors are conserved regulators of fungal mating. These genes, however, lose function frequently among Colletotrichum species, indicating a possibility that pheromone signaling is dispensable for Colletotrichum sexual reproduction. We have identified two putative pheromone-receptor pairs (PPG1:PRE2, PPG2:PRE1) in C. fructicola, a species that exhibits plus-to-minus mating type switching and plus-minus-mediated mating line development. Here, we report the generation and characterization of gene deletion mutants for all four genes in both plus and minus strain backgrounds. Single gene deletion of pre1 or pre2 had no effect on sexual development whereas their double deletion caused self-sterility in both the plus and minus strains. Moreover, double deletion of pre1 and pre2 caused female-sterility in plus-minus outcrossing. Double deletion of pre1 and pre2, however, did not inhibit perithecial differentiation or plus-minus mediated enhancement of perithecial differentiation. Contrary to the results with pre1 and pre2, double deletion of ppg1 and ppg2 had no effect on sexual compatibility, development or fecundity. We concluded that pre1 and pre2 coordinately regulate C. fructicola mating by recognizing novel signal molecule(s) distinct from canonical Ascomycota pheromones. The contrasting importance between pheromone receptors and their cognate pheromones highlights the complicated nature of sex regulation in Colletotrichum fungi" |
| Keywords: | Fungal Pathogens Genetics; |
| Notes: | "PublisherYao, Liqiang Kong, Yuanyuan Yang, Wenrui Tian, Huanhuan Meng, Xiangchen Zhao, Xuemei Zhang, Rong Sun, Guangyu Rollins, Jeffrey A Liang, Xiaofei eng 2023/05/04 Phytopathology. 2023 May 4. doi: 10.1094/PHYTO-11-22-0436-R" |
