| Title: | "Rapid identification of Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei in foods by solid phase microextraction coupled with gas chromatography-mass spectrometry" |
| Author(s): | Wang Y; Liu S; Pu Q; Li Y; Wang X; Jiang Y; Yang D; Yang Y; Yang J; Sun C; |
| Address: | "West China School of Public Health, Sichuan University, Chengdu 610041, China; Chongqing Center for Disease Control and Prevention, Chongqing 400042, China. West China School of Public Health, Sichuan University, Chengdu 610041, China. West China School of Public Health, Sichuan University, Chengdu 610041, China; Provincial Key Laboratory for Food Safety Monitoring and Risk Assessment of Sichuan, Chengdu 610041, China. Chengdu Center for Disease Control and Prevention, Chengdu 610041, China. Sichuan Center for Disease Control and Prevention, Chengdu 610041, China. West China School of Public Health, Sichuan University, Chengdu 610041, China; Provincial Key Laboratory for Food Safety Monitoring and Risk Assessment of Sichuan, Chengdu 610041, China. Electronic address: sunchj@scu.edu.cn" |
| DOI: | 10.1016/j.foodchem.2018.04.088 |
| ISSN/ISBN: | 1873-7072 (Electronic) 0308-8146 (Linking) |
| Abstract: | "A novel approach for rapid identification of three foodborne pathogens including Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei in foods by solid phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was established. After cultivation 24, 18 and 20?ª+h for Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei, respectively, the microbial volatile organic compounds (MVOCs) were extracted with a SPME device equipped with divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) coated fibers. The DB-1701P column was applied for separation of MVOCs. A total of 17, 13 and 14 volatile organic compounds were identified as characteristic MVOCs of Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei, respectively. Similarity of the MVOC chromatographic fingerprints for the bacteria were calculated and compared, and the results showed that the established method is stable, reproducible, accurate and has the potential to identify the three bacteria in food samples" |
| Keywords: | Dimethylpolysiloxanes/chemistry Food Microbiology/*methods Gas Chromatography-Mass Spectrometry/*methods Polyvinyls/chemistry Shigella sonnei/isolation & purification Solid Phase Microextraction/instrumentation/*methods Staphylococcus aureus/isolation & p; |
| Notes: | "MedlineWang, Yu Liu, Sijing Pu, Qikang Li, Yongxin Wang, Xixi Jiang, Yang Yang, Danni Yang, Yi Yang, Jinling Sun, Chengjun eng England 2018/05/13 Food Chem. 2018 Oct 1; 262:7-13. doi: 10.1016/j.foodchem.2018.04.088. Epub 2018 Apr 23" |
