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Food Chem (Oxf)

Title:		Transcriptome sequencing and gas chromatography-mass spectrometry analyses provide insights into beta-caryophyllene biosynthesis in Brassica campestris
Author(s):		Wang H; Zong C; Bai A; Yuan S; Li Y; Yu Z; Tian R; Liu T; Hou X; Li Y;
Address:		"National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, China. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China"
Journal Title:		Food Chem (Oxf)
Year:		2022
Volume:		20220813
Issue:
Page Number:		100129 -
DOI:		10.1016/j.fochms.2022.100129
ISSN/ISBN:		2666-5662 (Electronic) 2666-5662 (Linking)
Abstract:		"Sesquiterpenes are important defensive secondary metabolites and aroma components. However, limited information is available on the mechanism of sesquiterpene formation and composition in the non-heading Chinese cabbage (NHCC) leaf. Therefore, headspace solid-phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) combined with transcriptome analysis was used to study the mechanism of volatile organic compound formation. A total of 26 volatile organic compounds were identified in two NHCC cultivars 'SZQ' and 'XQC' and their F1 hybrids. Among these, sesquiterpene beta-caryophyllene was identified only in 'XQC' and F1. Five genes encoding caryophyllene synthase were identified. The candidate beta-caryophyllene synthase genes BcTPSa11 and BcTPSa21 had high expression levels only in 'XQC' and F1. In addition, several transcription factors of MYB-related, MYB, bHLH, and AP2/ERF families were identified by co-expression, suggesting that they regulate beta-caryophyllene biosynthesis. Our results provide a molecular basis for sesquiterpene biosynthesis as well as insights into the regulatory network of beta-caryophyllene in NHCC"
Keywords:		Hs-spme-gc/ms Non-heading Chinese cabbage Transcriptome analysis beta-Caryophyllene;
Notes:		"PubMed-not-MEDLINEWang, Haibin Zong, Chen Bai, Aimei Yuan, Shuilin Li, Yan Yu, Zhanghong Tian, Ruiping Liu, Tongkun Hou, Xilin Li, Ying eng England 2022/09/06 Food Chem (Oxf). 2022 Aug 13; 5:100129. doi: 10.1016/j.fochms.2022.100129. eCollection 2022 Dec 30"