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J Chem Ecol

Title:		Cytochromes P450 Preferentially Expressed in Antennae of the Mountain Pine Beetle
Author(s):		Chiu CC; Keeling CI; Bohlmann J;
Address:		"Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, B.C., V6T 1Z4, Canada. Department of Botany, University of British Columbia, 6270 University Blvd, Vancouver, B.C., V6T 1Z4, Canada. Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055 rue du P.E.P.S., Stn. Sainte-Foy, P.O. Box 10380, QC, Quebec, G1V 4C7, Canada. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, B.C., V6T 1Z4, Canada. bohlmann@msl.ubc.ca. Department of Botany, University of British Columbia, 6270 University Blvd, Vancouver, B.C., V6T 1Z4, Canada. bohlmann@msl.ubc.ca. Department of Forest and Conservation Sciences, University of British Columbia, 2441 Main Mall, Vancouver, B.C., V6T 1Z4, Canada. bohlmann@msl.ubc.ca"
Journal Title:		J Chem Ecol
Year:		2019
Volume:		20180725
Issue:		2
Page Number:		178 - 186
DOI:		10.1007/s10886-018-0999-0
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"The mountain pine beetle (MPB, Dendroctonus ponderosae) is a forest insect pest endemic to western North America. During dispersal and host colonization, MPB identify suitable host trees by olfaction of monoterpene volatiles, contend with host terpene defenses, and communicate with conspecifics using terpenoid and other pheromones. Cytochromes P450 (P450s) have been proposed to function in MPB olfaction, terpene detoxification, and pheromone biosynthesis. Here, we identified P450s that were abundant in the antennae transcriptome. Analysis of transcript levels across different life stages and tissues in females and males showed additional expression of most of these P450s in the midgut or fat body. These expression profiles suggest specific or overlapping functions in olfaction, detoxification, and pheromone biosynthesis"
Keywords:		Animals Arthropod Antennae/*metabolism Coleoptera/growth & development/*metabolism Cytochrome P-450 Enzyme System/genetics/*metabolism Fat Body/metabolism Female Life Cycle Stages Male Pheromones/chemistry/metabolism RNA/isolation & purification/metabolis;
Notes:		"MedlineChiu, Christine C Keeling, Christopher I Bohlmann, Joerg eng NSERC Discovery/Natural Sciences and Engineering Research Council of Canada/ TRIA Net/Natural Sciences and Engineering Research Council of Canada/ 2018/07/26 J Chem Ecol. 2019 Feb; 45(2):178-186. doi: 10.1007/s10886-018-0999-0. Epub 2018 Jul 25"