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J Insect Physiol

Title:		"The sensilla trichodea-biased EoblPBP1 binds sex pheromones and green leaf volatiles in Ectropis obliqua Prout, a geometrid moth pest that uses Type-II sex pheromones"
Author(s):		Sun L; Wang Q; Zhang Y; Tu X; Yan Y; Wang Q; Dong K; Zhang Y; Xiao Q;
Address:		"Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. Electronic address: liangsun@tricaas.com. College of Agriculture and Food Science, Zhejiang A & F University, Hangzhou, China. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. Electronic address: xqtea@mail.tricaas.com"
Journal Title:		J Insect Physiol
Year:		2019
Volume:		20190419
Issue:
Page Number:		17 - 24
DOI:		10.1016/j.jinsphys.2019.04.005
ISSN/ISBN:		1879-1611 (Electronic) 0022-1910 (Linking)
Abstract:		"Pheromone-binding proteins (PBPs) are considered to play critical roles in sex pheromone detection. Lepidopteran moths can be divided into two taxa, those that use Type-I sex pheromones, such as C10-C18 unsaturated aldehydes, alcohols and acetates, and those that use Type-II pheromones, which are C17-C23 polyunsaturated hydrocarbons and their epoxide derivatives. To date, nearly all the characterized PBPs have been reported in moths with Type-I sex pheromones, and the physiological functions of PBPs in moths that use Type-II sex pheromones remains unclear. In the present study we functionally examine EoblPBP1 in Ectropis obliqua Prout, an important geometrid moth pest that uses Type-II sex pheromones. The phylogenetic analysis of the sequence indicated that EoblPBP1 clustered together with ScerPBP1, a geometrid PBP for detecting Type-II sex pheromones. Scanning electron microscopy showed that E. obliqua moths of both sexes mainly had six types of antennal sensilla, including two types of sensilla trichodea, Str-I and Str-II, sensilla basiconica (Sba), sensilla styloconica (Sst), sensilla chaetica (Sch) and sensilla auricillica (Sau). Of these, Str-I was confirmed to be male moth-specific and had five different subtypes. Fluorescence in situ hybridization revealed that EoblPBP1 was primarily expressed at the base of Str-I. A comparative binding assay showed that recombinant EoblPBP1 bound three sex pheromone components of E. obliqua, demonstrating its involvement in the detection of Type-II sex pheromones. Besides, EoblPBP1 also highly bound unsaturated acetates pheromones and the green leaf volatiles. These results indicate that PBP1 is associated with detecting Type-II sex pheromones in E. obliqua but cannot differentiate Type-II sex pheromones from Type-I sex pheromones or green leaf volatiles. Our findings provide a foundation for further study on molecular basis of Type-II sex pheromone recognition in lepidopteran moths"
Keywords:		"Animals Arthropod Antennae/metabolism/ultrastructure Carrier Proteins/*metabolism Female Insect Proteins/*metabolism Male Microscopy, Electron, Scanning Microscopy, Electron, Transmission Moths/*metabolism/ultrastructure Phylogeny Plant Leaves/chemistry S;"
Notes:		"MedlineSun, Liang Wang, Qian Zhang, Yuxing Tu, Xiaohui Yan, Yuting Wang, Qi Dong, Kun Zhang, Yongjun Xiao, Qiang eng Research Support, Non-U.S. Gov't England 2019/04/23 J Insect Physiol. 2019 Jul; 116:17-24. doi: 10.1016/j.jinsphys.2019.04.005. Epub 2019 Apr 19"