| Title: | "Phenotypic plasticity in plant defense across life stages: Inducibility, transgenerational induction, and transgenerational priming in wild radish" |
| Author(s): | Sobral M; Sampedro L; Neylan I; Siemens D; Dirzo R; |
| Address: | "Departamento de Bioloxia Funcional, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. Department of Biology, Stanford University, Stanford, CA 94305. Mision Bioloxica de Galicia, Consejo Superior de Investigaciones Cientificas, 36143 Pontevedra, Spain. Department of Evolution and Ecology, Center for Population Biology, University of California, Davis, CA 95616. Biology Department, Black Hills State University, Spearfish, SD 57799. Department of Biology, Stanford University, Stanford, CA 94305; rdirzo@stanford.edu" |
| ISSN/ISBN: | 1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking) |
| Abstract: | "As they develop, many plants deploy shifts in antiherbivore defense allocation due to changing costs and benefits of their defensive traits. Plant defenses are known to be primed or directly induced by herbivore damage within generations and across generations by long-lasting epigenetic mechanisms. However, little is known about the differences between life stages of epigenetically inducible defensive traits across generations. To help fill this knowledge gap, we conducted a multigenerational experiment to determine whether defense induction in wild radish plants was reflected in chromatin modifications (DNA methylation); we then examined differences between seedlings and reproductive plants in current and transgenerational plasticity in chemical (glucosinolates) and physical (trichomes) defenses in this species. Herbivory triggered genome methylation both in targeted plants and their offspring. Within one generation, both defenses were highly inducible at the seedling stage, but only chemical defenses were inducible in reproductive plants. Across generations, herbivory experienced by mother plants caused strong direct induction of physical defenses in their progeny, with effects lasting from seedling to reproductive stages. For chemical defenses, however, this transgenerational induction was evident only in adults. Transgenerational priming was observed in physical and chemical defenses, particularly in adult plants. Our results show that transgenerational plasticity in plant defenses in response to herbivore offense differs for physical and chemical defense and changes across plant life stages" |
| Keywords: | "Adaptation, Physiological/*genetics Animals DNA/genetics DNA Methylation Epigenesis, Genetic Herbivory/*physiology Larva/physiology Lepidoptera/*physiology Phenotype Plant Leaves Raphanus/*genetics/*growth & development ecology epigenetics evolution herbi;" |
| Notes: | "MedlineSobral, Mar Sampedro, Luis Neylan, Isabelle Siemens, David Dirzo, Rodolfo eng Research Support, Non-U.S. Gov't 2021/08/15 Proc Natl Acad Sci U S A. 2021 Aug 17; 118(33):e2005865118. doi: 10.1073/pnas.2005865118" |
