| Title: | The effects of different biochars on Caenorhabditis elegans and the underlying transcriptomic mechanisms |
| Author(s): | Chen Y; Wang X; Li J; Wang Z; Song T; Lai X; Zhang G; Ruan W; |
| Address: | "Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, China. College of Agronomy, Shenyang Agricultural University, Shenyang, China. College of Resources and Environment, Northeast Agricultural University, Harbin, China. Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing, China. College of Life Sciences, Nankai University, Tianjin, China" |
| DOI: | 10.1371/journal.pone.0284348 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Different biochars have diverse properties, with ambiguous effects on soil nematodes. This study investigated how aspen sawdust (ABC), bamboo powder (BBC), maize straw (MBC) and peanut-shell biochars (PBC) affected Caenorhabditis elegans via culture assays and RNA-seq analysis. The results showed that biochars derived from different agricultural materials varied significantly in physicochemical properties, and PBC produced more volatile organic compounds (VOCs) to attract C. elegans than ABC, BBC and MBC. Moreover, worms in ABC experienced the worst outcomes, while worms in PBC experienced milder impacts. Nematode body length decreased to 724.6 mum, 784.0 mum and 799.7 mum on average in ABC, BBC and MBC, respectively, compared to the control (1052 mum) and PBC treatments (960 mum). The brood size in ABC, MBC, BBC and PBC decreased 41.1%, 39.4%, 39.2% and 19.1% compared to the control, respectively. Furthermore, the molecular mechanisms of biochar-induced developmental effects on C. elegans were explored. Although several differentially expressed genes (DEGs) were different among the four biochars, worm phenotypic changes were mainly related to col genes (col-129; col-140; col-40; col-184), bli-6, sqt-3, perm-2/4, cdk-8, daf-16 and sod-1/2/5, which are associated with cuticle collagen synthesis, eggshell formation in postembryonic growth and rhythmic processes. Our study suggests that different properties of biochars could be crucial to soil nematodes, as well as the worms' biochemical changes are important for the health in agriculture soil" |
| Keywords: | Animals *Transcriptome *Caenorhabditis elegans/genetics Gene Expression Profiling Arachis Soil; |
| Notes: | "MedlineChen, Yixuan Wang, Xinrui Li, Jie Wang, Zhiwen Song, Tingting Lai, Xin Zhang, Guilong Ruan, Weibin eng Research Support, Non-U.S. Gov't 2023/09/22 PLoS One. 2023 Sep 22; 18(9):e0284348. doi: 10.1371/journal.pone.0284348. eCollection 2023" |
