| Title: | Phenotypic detection of quorum sensing inhibition in Pseudomonas aeruginosa pyoverdine and swarming by volatile organic products |
| Author(s): | Ramirez-Rueda Y; Salvador MJ; |
| Address: | "Department of Plant Biology, PPG BTPB & PPG BV, Institute of Biology, University of Campinas - UNICAMP, Postal Box 6109, Campinas - SP 13083-970, Brazil. Faculty of Health Sciences. Pedagogical & Technological University of Colombia, Postal Box 0387437173 Tunja, Calle 24 N degrees 5-63, Colombia" |
| ISSN/ISBN: | 1746-0921 (Electronic) 1746-0913 (Linking) |
| Abstract: | "Aim: To determine phenotypically the anti quorum-sensing (QS) activity of 30 volatile organic products (VOPs) through the inhibition of swarming motility and pyoverdine production in Pseudomonas aeruginosa. Materials & methods: Twenty-four essential oils and six small volatile organic compounds randomly selected were screened for their anti-QS activity by violacein inhibition on Chromobacterium violaceum. The VOPs with positive results were subsequently evaluated for swarming motility and pyoverdine production on P. aeruginosa determining the colony diameter and fluorescence under UV light, respectively. Results: Fifty percent of VOPs tested showed strong violacein inhibition, 40% presented anti-swarming activity and 33% inhibited pyoverdine production. Conclusion: Our data demonstrate that VOPs have a great potential to inhibit virulence factors mediated by QS in P. aeruginosa" |
| Keywords: | "Anti-Bacterial Agents/*pharmacology Chromobacterium/drug effects/physiology Oils, Volatile/pharmacology Plant Extracts/*pharmacology Plants/chemistry Pseudomonas aeruginosa/cytology/*drug effects/physiology Pyocyanine/*biosynthesis Quorum Sensing/*drug ef;" |
| Notes: | "MedlineY Ramirez-Rueda, Roman Salvador, Marcos J eng Research Support, Non-U.S. Gov't England 2020/09/22 Future Microbiol. 2020 Aug; 15:1147-1156. doi: 10.2217/fmb-2020-0033" |
