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J Agric Food Chem

Title:		Spatially Resolved Headspace Extractions of Trace-Level Volatiles from Planar Surfaces for High-Throughput Quantitation and Mass Spectral Imaging
Author(s):		Rafson JP; Bee MY; Sacks GL;
Address:		"Department of Food Science , Cornell University , Stocking Hall, Ithaca , New York 14853 , United States"
Journal Title:		J Agric Food Chem
Year:		2019
Volume:		20190415
Issue:		50
Page Number:		13840 - 13847
DOI:		10.1021/acs.jafc.9b01091
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"The use of headspace thin-film microextraction devices (SPMESH) for parallel extraction of trace-level volatiles prior to direct analysis in real-time mass spectrometry (DART-MS) has been reported previously, in which volatiles were extracted from samples in multi-well plates. In this report, we demonstrate that headspace extraction of volatiles by SPMESH sheets can be performed directly from planar surfaces. When coupled with DART-MS, this approach yields volatile mass spectral images with at least 4 mm resolution. When samples were spotted onto general-purpose silica gel thin-layer chromatography (TLC) plates, the SPMESH extraction could reach equilibrium within 2-4 min and 48 samples could be extracted and analyzed in 14 min. Because volatilization of analytes from TLC plates was very rapid, SPMESH extraction was delayed by the addition of 5% polyethylene glycol. Good linearity was achieved in the microgram per liter to milligram per liter range for four odorants (3-isobutyl-2-methoxypyrazine, linalool, methyl anthranilate, and o-aminoacetophenone) in several matrices (water, 10% ethanol, juice, and grape macerate) using 5 muL sample sizes. Detection limits as low as 50 pg/spot (10 mug/L in grape macerate) could be achieved. In contrast to many reports on headspace solid-phase microextraction, negligible matrix effects were observed for ethanol and grape macerates compared to water. SPMESH can preserve volatile images from planar surfaces, and SPMESH-DART-MS from TLC plates is well-suited for rapid trace volatile analysis, especially with small sample sizes"
Keywords:		"Chromatography, Thin Layer/*methods Mass Spectrometry/methods Solid Phase Microextraction/*methods Vitis/*chemistry Volatile Organic Compounds/*chemistry/*isolation & purification Dart ambient ionization high-throughput mass spectral imaging volatile anal;"
Notes:		"MedlineRafson, Jessica P Bee, Madeleine Y Sacks, Gavin L eng Evaluation Study 2019/04/05 J Agric Food Chem. 2019 Dec 18; 67(50):13840-13847. doi: 10.1021/acs.jafc.9b01091. Epub 2019 Apr 15"