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Insect Mol Biol

Title:		The sensory neurone membrane protein SNMP1 contributes to the sensitivity of a pheromone detection system
Author(s):		Pregitzer P; Greschista M; Breer H; Krieger J;
Address:		"Institute of Physiology, University of Hohenheim, Stuttgart, Germany"
Journal Title:		Insect Mol Biol
Year:		2014
Volume:		20140721
Issue:		6
Page Number:		733 - 742
DOI:		10.1111/imb.12119
ISSN/ISBN:		1365-2583 (Electronic) 0962-1075 (Linking)
Abstract:		"Male moths detect female-released sex pheromones with extraordinary sensitivity. The remarkable sensory ability is based on a cooperative interplay of pheromone binding proteins in the lymph of hair-like sensilla trichodea and pheromone receptors in the dendrites of sensory neurones. Here we examined whether in Heliothis virescens the so-called 'sensory neurone membrane protein 1' (SNMP1) may contribute to responsiveness to the pheromone component, (Z)-11-hexadecenal (Z11-16:Ald). By means of immunohistochemistry and in situ hybridization we demonstrated that SNMP1 is in fact present in cells expressing the Z11-16:Ald receptor HR13 and the dendrites of sensory neurones. To assess a possible function of SNMP1 we monitored the responsiveness of cell lines that expressed HR13 alone or the combination SNMP1/HR13 to stimulation with Z11-16:Ald by calcium imaging. It was found that SNMP1/HR13 cells were 1000-fold more sensitive to pheromone stimulation compared with HR13 cells. In contrast, cells that expressed HR13 and the non-neuronal SNMP2-type showed no change in pheromone sensitivity. Overall, our reconstitution experiments demonstrate that the presence of SNMP1 significantly increases the HR13-based responsiveness of cells to Z11-16:Ald, suggesting that SNMP1 also contributes to the response of the antennal neurones and thus to the remarkable sensitivity of the pheromone detection system"
Keywords:		"Aldehydes/metabolism Animals Arthropod Antennae Carrier Proteins In Situ Hybridization Male Membrane Proteins/metabolism Moths/*physiology Nerve Tissue Proteins/metabolism Olfactory Receptor Neurons/*metabolism Receptors, Pheromone/genetics/*metabolism Se;"
Notes:		"MedlinePregitzer, P Greschista, M Breer, H Krieger, J eng Research Support, Non-U.S. Gov't England 2014/07/23 Insect Mol Biol. 2014 Dec; 23(6):733-42. doi: 10.1111/imb.12119. Epub 2014 Jul 21"