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Chem Senses

Title:		A photoaffinity-labeled green leaf volatile compound 'tricks' highly selective and sensitive insect olfactory receptor neurons
Author(s):		Nikonov AA; Valiyaveettil JT; Leal WS;
Address:		"Laboratory of Chemical Prospecting, National Institute of Sericultural and Entomological Science, 1-2 Ohwashi, Tsukuba 305-8634, Japan and. Department of Entomology, University of California at Davis, Davis, CA 95616, USA"
Journal Title:		Chem Senses
Year:		2001
Volume:		26
Issue:		1
Page Number:		49 - 54
DOI:		10.1093/chemse/26.1.49
ISSN/ISBN:		0379-864X (Print) 0379-864X (Linking)
Abstract:		"The sex pheromone of the scarab beetle, Phyllopertha diversa, is emitted by females and specifically detected by olfactory receptor neurons in the male and female antennae. Single sensillum recordings showed that, in contrast to the less sensitive pheromone sensilla in females, olfactory receptor neurons in the male antennae had a low threshold (1 ng), which rivals those of moths. The male and female antennae also possessed olfactory receptor neurons specific for the detection of floral and green leaf volatile compounds. Detectors for the green leaf volatile (Z)-3-hexenyl acetate had a threshold (10 pg) far below the sensitivity of the pheromone-detecting machinery. In addition, these neurons showed a remarkable selectivity even when challenged with related compounds at 10000-fold higher concentrations. Surprisingly, a diazo analog, (Z)-3-hexenyl diazoacetate, elicited slightly higher nervous activity than the natural ligand in the neurons specific and selective for (Z)-3-hexenyl acetate. The inability of the green leaf volatile-detecting machinery to discriminate the photoaffinity-labeled compound from the natural product indicates that the synthetic ligand interacts with odorant-binding protein, odorant receptor and odorant-degrading enzyme as does the cognate ligand"
Keywords:		Acetates/pharmacology Affinity Labels Animals Coleoptera/*physiology Female Ligands Male Olfactory Receptor Neurons/*drug effects/*physiology Plant Extracts/chemistry/*pharmacology Sex Attractants/pharmacology/physiology;
Notes:		"MedlineNikonov, A A Valiyaveettil, J T Leal, W S eng Research Support, Non-U.S. Gov't England 2000/12/22 Chem Senses. 2001 Jan; 26(1):49-54. doi: 10.1093/chemse/26.1.49"