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FEMS Microbiol Lett

Title:		Targeted disruption of the PD78 gene (traF) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis
Author(s):		Nakayama J; Clewell DB; Suzuki A;
Address:		"Department of Applied Biological Chemistry, University of Tokyo, Japan"
Journal Title:		FEMS Microbiol Lett
Year:		1995
Volume:		128
Issue:		3
Page Number:		283 - 288
DOI:		10.1111/j.1574-6968.1995.tb07537.x
ISSN/ISBN:		0378-1097 (Print) 0378-1097 (Linking)
Abstract:		"Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF. The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level"
Keywords:		"Bacterial Proteins/*genetics Bacteriocins/*genetics Blotting, Southern *Conjugation, Genetic/drug effects Enterococcus faecalis/*genetics Escherichia coli/genetics Genes, Bacterial/genetics Mutagenesis, Insertional Pheromones/pharmacology Plasmids/*geneti;"
Notes:		"MedlineNakayama, J Clewell, D B Suzuki, A eng Research Support, Non-U.S. Gov't England 1995/05/15 FEMS Microbiol Lett. 1995 May 15; 128(3):283-8. doi: 10.1111/j.1574-6968.1995.tb07537.x"