| Title: | Studies on the yeast alpha-mating factor: a model for mammalian peptide hormones |
| Author(s): | Naider F; Gounarides J; Xue CB; Bargiota E; Becker JM; |
| Address: | "Department of Chemistry, College of Staten Island, City University of New York 10301" |
| ISSN/ISBN: | 0006-3525 (Print) 0006-3525 (Linking) |
| Abstract: | "Small peptides initiate sexual conjugation in the yeast Saccharomyces cerevisiae and this phenomenon is an ideal paradigm for studying the mode of action of mammalian peptide hormones. 1H-nmr spectroscopy was used to examine the conformation of linear and cyclic analogues of the alpha-factor (WHWLQLKPGQPMY) in aqueous solution. In all cases peptides that exhibit nmr parameters expected for a type II beta-turn have higher biological activities than those that do not appear to assume this conformation. Based on a simple model for the interaction of the pheromone with its receptor, we prepared fragments of the alpha-factor. Several of these fragments either antagonize or potentiate the activity of the alpha-factor. The latter represent the first example of peptide fragments that synergize the activity of the parent pheromone" |
| Keywords: | "Amino Acid Sequence Animals Fungal Proteins/antagonists & inhibitors/*chemistry Hormones/*chemistry/physiology Magnetic Resonance Spectroscopy Mammals Mating Factor Models, Biological Molecular Sequence Data Peptides/antagonists & inhibitors/*chemistry/ph;" |
| Notes: | "MedlineNaider, F Gounarides, J Xue, C B Bargiota, E Becker, J M eng GM 22086/GM/NIGMS NIH HHS/ GM 22087/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1992/04/01 Biopolymers. 1992 Apr; 32(4):335-9. doi: 10.1002/bip.360320407" |
