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Contact Dermatitis

Title:		Stability of selected volatile contact allergens in different patch test chambers under different storage conditions
Author(s):		Mose KF; Andersen KE; Christensen LP;
Address:		"Department of Dermatology and Allergy Centre, Odense University Hospital, DK-5000 Odense C, Denmark"
Journal Title:		Contact Dermatitis
Year:		2012
Volume:		66
Issue:		4
Page Number:		172 - 179
DOI:		10.1111/j.1600-0536.2011.02035.x
ISSN/ISBN:		1600-0536 (Electronic) 0105-1873 (Linking)
Abstract:		"BACKGROUND: Patch test preparations of volatile substances may evaporate during storage, thereby giving rise to reduced patch test concentrations. OBJECTIVES: To investigate the stability of selected acrylates/methacrylates and fragrance allergens in three different test chambers under different storage conditions. METHODS: Petrolatum samples of methyl methacrylate (MMA), 2-hydroxyethyl methacrylate (2-HEMA), 2-hydroxypropyl acrylate (2-HPA), cinnamal and eugenol in patch test concentrations were stored in three different test chambers (IQ chamber, IQ Ultimate, and Van der Bend(R) transport container) at room temperature and in a refrigerator. The samples were analysed in triplicate with high-performance liquid chromatography. RESULTS: The decrease in concentration was substantial for all five allergens under both storage conditions in IQ chamber and IQ Ultimate, with the exception of 2-HEMA during storage in the refrigerator. For these two chamber systems, the contact allergen concentration dropped below the stability limit in the following order: MMA, cinnamal, 2-HPA, eugenol, and 2-HEMA. In the Van der Bend(R) transport container, the contact allergens exhibited acceptable stability under both storage conditions, whereas MMA and 2-HPA required cool storage for maintenance of the limit. CONCLUSION: The Van der Bend(R) transport container was the best device for storage of samples of volatile contact allergens"
Keywords:		"Acrolein/*analogs & derivatives/chemistry Acrylates/*chemistry Allergens/chemistry Chromatography, High Pressure Liquid Dermatitis, Allergic Contact/*diagnosis Drug Stability Drug Storage Eugenol/*chemistry Flavoring Agents/chemistry Humans Methacrylates/;"
Notes:		"MedlineMose, Kristian F Andersen, Klaus E Christensen, Lars Porskjaer eng England 2012/03/13 Contact Dermatitis. 2012 Apr; 66(4):172-9. doi: 10.1111/j.1600-0536.2011.02035.x"