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Forensic Sci Int

Title:		Qualitative and quantitative analysis of a group of volatile organic compounds in biological samples by HS-GC/FID: application in practical cases
Author(s):		Monteiro C; Franco JM; Proenca P; Castanera A; Claro A; Vieira DN; Corte-Real F;
Address:		"National Institute of Legal Medicine and Forensic Sciences-Centre Branch, Largo da Se Nova, 3000-213 Coimbra, Portugal; CENCIFOR-Forensic Sciences Centre, Largo da Se Nova, 3000-213 Coimbra, Portugal. Electronic address: carla.monteiro@dcinml.mj.pt. National Institute of Legal Medicine and Forensic Sciences-Centre Branch, Largo da Se Nova, 3000-213 Coimbra, Portugal; CENCIFOR-Forensic Sciences Centre, Largo da Se Nova, 3000-213 Coimbra, Portugal. CENCIFOR-Forensic Sciences Centre, Largo da Se Nova, 3000-213 Coimbra, Portugal; National Institute of Legal Medicine and Forensic Sciences-South Branch, Rua Manuel Bento de Sousa 3, 1150-219 Lisboa, Portugal. National Institute of Legal Medicine and Forensic Sciences-Centre Branch, Largo da Se Nova, 3000-213 Coimbra, Portugal. National Institute of Legal Medicine and Forensic Sciences-Centre Branch, Largo da Se Nova, 3000-213 Coimbra, Portugal; CENCIFOR-Forensic Sciences Centre, Largo da Se Nova, 3000-213 Coimbra, Portugal; Faculty of Medicine-University of Coimbra, Largo da Se Nova, 3000-213 Coimbra, Portugal"
Journal Title:		Forensic Sci Int
Year:		2014
Volume:		20140725
Issue:
Page Number:		137 - 143
DOI:		10.1016/j.forsciint.2014.07.016
ISSN/ISBN:		1872-6283 (Electronic) 0379-0738 (Linking)
Abstract:		"A simple and sensitive procedure, using n-propanol as internal standard (IS), was developed and validated for the qualitative and quantitative analysis of a group of 11 volatile organic substances with different physicochemical properties (1-butanol, 2-propanol, acetaldehyde, ethyl acetate, acetone, acetonitrile, chloroform, diethyl ether, methanol, toluene and p-xylene) in whole blood, urine and vitreous humor. Samples were prepared by dilution with an aqueous solution of internal standard followed by Headspace Gas Chromatography with a Flame-ionization Detector (HS GC-FID) analysis. Chromatographic separation was performed using two capillary columns with different polarities (DB-ALC2: 30mx0.320mmx1.2mum and DB-ALC1: 30mx0.320mmx1.8mum), thus providing a change in the retention and elution order of volatiles. This dual column confirmation increases the specificity, since the risk of another substance co-eluting at the same time in both columns is very small. The method was linear from 5 to 1000mg/L for toluene and p-xylene, 50-1000mg/L for chloroform, and 50-2000mg/L for the remaining substances, with correlation coefficients of over 0.99 for all compounds. The limits of detection (LOD) ranged 1 to 10mg/L, while the limits of quantification (LOQ) ranged from 2 to 31mg/L. The intra-day precision (CV<6.4%), intermediate precision (CV<7.0%) and accuracy (relative error +/-10%) of the method were in conformity with the criteria normally accepted in bioanalytical method validation. The method developed has been applied to forensic cases, with the advantages that it uses a small sample volume and does not require any extraction procedure as it makes use of a headspace injection technique"
Keywords:		"Chromatography, Gas/*methods *Flame Ionization Humans Limit of Detection Vitreous Body/chemistry Volatile Organic Compounds/*analysis Forensic toxicology Hs gc-fid Validation Volatile organic compound;"
Notes:		"MedlineMonteiro, C Franco, J M Proenca, P Castanera, A Claro, A Vieira, D N Corte-Real, F eng Validation Study Ireland 2014/08/16 Forensic Sci Int. 2014 Oct; 243:137-43. doi: 10.1016/j.forsciint.2014.07.016. Epub 2014 Jul 25"