| Title: | Sublethal Exposure Effects of the Neonicotinoid Clothianidin Strongly Modify the Brain Transcriptome and Proteome in the Male Moth Agrotis ipsilon |
| Author(s): | Meslin C; Bozzolan F; Braman V; Chardonnet S; Pionneau C; Francois MC; Severac D; Gadenne C; Anton S; Maibeche M; Jacquin-Joly E; Siaussat D; |
| Address: | "Departement Ecologie Sensorielle, Institut d'Ecologie et des Sciences de l'Environnement de Paris (iEES-Paris), Sorbonne Universite, INRAE, CNRS, IRD, UPEC, Universite de Paris, 75005 Paris, France. Departement Ecologie Sensorielle, Institut d'Ecologie et des Sciences de l'Environnement de Paris (iEES-Paris), Sorbonne Universite, INRAE, CNRS, IRD, UPEC, Universite de Paris, 78026 Versailles, France. Plateforme Post-Genomique de la Pitie-Salpetriere (P3S), UMS 37 PASS, INSERM, Sorbonne Universite, 75013 Paris, France. MGX, BioCampus Montpellier, CNRS, INSERM, Universite de Montpellier, 34000 Montpellier, France. Institut de Genetique Environnement et Protection des Plantes IGEPP, INRAE, Institut Agro, Universite de Rennes, 49045 Angers, France" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "Insect pest management relies mainly on neurotoxic insecticides, including neonicotinoids such as clothianidin. The residual accumulation of low concentrations of these insecticides can have positive effects on target pest insects by enhancing various life traits. Because pest insects often rely on sex pheromones for reproduction and olfactory synaptic transmission is cholinergic, neonicotinoid residues could indeed modify chemical communication. We recently showed that treatments with low doses of clothianidin could induce hormetic effects on behavioral and neuronal sex pheromone responses in the male moth, Agrotis ipsilon. In this study, we used high-throughput RNAseq and proteomic analyses from brains of A. ipsilon males that were intoxicated with a low dose of clothianidin to investigate the molecular mechanisms leading to the observed hormetic effect. Our results showed that clothianidin induced significant changes in transcript levels and protein quantity in the brain of treated moths: 1229 genes and 49 proteins were differentially expressed upon clothianidin exposure. In particular, our analyses highlighted a regulation in numerous enzymes as a possible detoxification response to the insecticide and also numerous changes in neuronal processes, which could act as a form of acclimatization to the insecticide-contaminated environment, both leading to enhanced neuronal and behavioral responses to sex pheromone" |
| Keywords: | Agrotis ipsilon clothianidin hormesis pest insect proteomics transcriptomics; |
| Notes: | "PubMed-not-MEDLINEMeslin, Camille Bozzolan, Francoise Braman, Virginie Chardonnet, Solenne Pionneau, Cedric Francois, Marie-Christine Severac, Dany Gadenne, Christophe Anton, Sylvia Maibeche, Martine Jacquin-Joly, Emmanuelle Siaussat, David eng PHEROTOX/Agence Nationale de la Recherche/ Switzerland 2021/03/07 Insects. 2021 Feb 11; 12(2):152. doi: 10.3390/insects12020152" |
