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J Chem Ecol

Title:		Purine metabolizing capability of Enterobacter agglomerans affects volatiles production and attractiveness to Mexican fruit fly
Author(s):		Robacker DC; Lauzon CR;
Address:		"Crop Quality and Fruit Insects Research, ARS, USDA Weslaco, Texas 78596, USA. drobacker@weslaco.ars.usda.gov"
Journal Title:		J Chem Ecol
Year:		2002
Volume:		28
Issue:		8
Page Number:		1549 - 1563
DOI:		10.1023/a:1019920328062
ISSN/ISBN:		0098-0331 (Print) 0098-0331 (Linking)
Abstract:		"We investigated two strains of Enterobacter agglomerans that differ in their ability to metabolize uric acid for (1) attractiveness to sugar-fed Mexican fruit flies, and (2) production of volatile chemicals that may be responsible for the attractiveness. The two strains were cultured on a medium that contained uric acid as the primary nitrogen source to simulate bird feces, a natural substrate for this bacterium. Active cultures of both strains were more attractive than uninoculated uric acid medium to both sexes of sugar-fed flies in wind-tunnel bioassays. The uricase(+) strain was more attractive than the uricase(-) strain to males and to females <9 days old, but not to older females. Volatiles found by solid-phase microextraction in greater amounts in headspace above active cultures of both strains than above uninoculated medium were ammonia, dimethyldisulfide, 3-methylbutanol, 2-phenylethanol, 2,5-dimethylpyrazine, and trimethylpyrazine. The uricase(+) strain produced more ammonia, dimethyldisulfide, and trimethylpyrazine than the uricase(-) strain. An additional chemical, 3-hydroxybutanone, appears to be produced exclusively by the uricase(+) strain. The uricase(-) strain produced more 2-phenylethanol than the uricase(+) strain. Differences in volatiles are consistent with the generally greater attractiveness of the uricase(+) strain compared with the uricase(-) strain as ammonia, 3-hydroxybutanone, and trimethylpyrazine have been demonstrated attractive to sugar-fed Mexican fruit flies"
Keywords:		"Animals Chromatography, Gas Diptera/*physiology Enterobacter/*metabolism Female Male Pheromones/analysis/*biosynthesis Purines/*metabolism Volatilization;"
Notes:		"MedlineRobacker, David C Lauzon, Carol R eng 2002/10/10 J Chem Ecol. 2002 Aug; 28(8):1549-63. doi: 10.1023/a:1019920328062"