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FEMS Yeast Res

Title:		Use of a wine yeast deletion collection reveals genes that influence fermentation performance under low-nitrogen conditions
Author(s):		Peter JJ; Watson TL; Walker ME; Gardner JM; Lang TA; Borneman A; Forgan A; Tran T; Jiranek V;
Address:		"Department of Wine and Food Science, School of Agriculture Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia. The Australian Wine Research Institute, Waite Campus, Urrbrae, SA 5064, Australia. Australian Research Council Training Centre for Innovative Wine Production, The University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia"
Journal Title:		FEMS Yeast Res
Year:		2018
Volume:		18
Issue:		3
Page Number:		-
DOI:		10.1093/femsyr/foy009
ISSN/ISBN:		1567-1364 (Electronic) 1567-1356 (Linking)
Abstract:		"A deficiency of nitrogenous nutrients in grape juice can cause stuck and sluggish alcoholic fermentation, which has long been a problem in winemaking. Nitrogen requirements vary between wine yeast strains, and the ability of yeast to assimilate nitrogen depends on the nature and concentration of nitrogen present in the medium. In this study, a wine yeast gene deletion collection (1844 deletants in the haploid AWRI1631 background) was screened to identify genes whose deletion resulted in a reduction in the time taken to utilise all sugars when grown in a chemically defined grape juice medium supplemented with limited nitrogen (75 mg L-1 as a free amino acid mixture). Through micro-scale and laboratory-scale fermentations, 15 deletants were identified that completed fermentation in a shorter time than the wildtype (c.a. 15%-59% time reduction). This group of genes was annotated to biological processes including protein modification, transport, metabolism and ubiquitination (UBC13, MMS2, UBP7, UBI4, BRO1, TPK2, EAR1, MRP17, MFA2 and MVB12), signalling (MFA2) and amino acid metabolism (AAT2). Deletion of MFA2, encoding mating factor-a, resulted in a 55% decrease in fermentation duration. Mfa2Delta was chosen for further investigation to understand how this gene deletion conferred fermentation efficiency in limited nitrogen conditions"
Keywords:		"Amino Acids/metabolism Culture Media/chemistry Fermentation/*genetics *Gene Deletion *Genes, Fungal Lipoproteins/genetics Nitrogen/*metabolism Pheromones/genetics Saccharomyces cerevisiae/*genetics/physiology Saccharomyces cerevisiae Proteins/genetics Ubi;"
Notes:		"MedlinePeter, Josephine J Watson, Tommaso L Walker, Michelle E Gardner, Jennifer M Lang, Tom A Borneman, Anthony Forgan, Angus Tran, Tina Jiranek, Vladimir eng Research Support, Non-U.S. Gov't England 2018/02/10 FEMS Yeast Res. 2018 May 1; 18(3). doi: 10.1093/femsyr/foy009"