| Title: | "Sex pheromone recognition and characterization of three pheromone-binding proteins in the legume pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae)" |
| Author(s): | Mao A; Zhou J; Bin M; Zheng Y; Wang Y; Li D; Wang P; Liu K; Wang X; Ai H; |
| Address: | "Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore. Key Laboratory of Insect Resource Utilization &Sustainable Pest Management of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Pheromone-binding proteins (PBPs) are essential for the filtering, binding and transporting of sex pheromones across sensillum lymph to membrane-associated pheromone receptors of moths. In this study, three novel PBP genes were expressed in Escherichia coli to examine their involvement in the sex pheromone perception of Maruca vitrata. Fluorescence binding experiments indicated that MvitPBP1-3 had strong binding affinities with four sex pheromones. Moreover, molecular docking results demonstrated that six amino acid residues of three MvitPBPs were involved in the binding of the sex pheromones. These results suggested that MvitPBP1-3 might play critical roles in the perception of female sex pheromones. Additionally, the binding capacity of MvitPBP3 with the host-plant floral volatiles was high and was similar to that of MvitGOBP2. Furthermore, sequence alignment and docking analysis showed that both MvitGOBP2 and MvitPBP3 possessed an identical key binding site (arginine, R130/R140) and a similar protein pocket structure around the binding cavity. Therefore, we hypothesized that MvitPBP3 and MvitGOBP2 might have synergistic roles in binding different volatile ligands. In combination, the use of synthetic sex pheromones and floral volatiles from host-plant may be used in the exploration for more efficient monitoring and integrated management strategies for the legume pod borer in the field" |
| Keywords: | Animals Binding Sites Carrier Proteins/*chemistry/genetics/metabolism Escherichia coli/chemistry/genetics/metabolism Female Gene Expression Insect Proteins/*chemistry/genetics/metabolism Lepidoptera/*chemistry/genetics/metabolism Male *Molecular Docking S; |
| Notes: | "MedlineMao, Aping Zhou, Jing Bin Mao Zheng, Ya Wang, Yufeng Li, Daiqin Wang, Pan Liu, Kaiyu Wang, Xiaoping Ai, Hui eng Research Support, Non-U.S. Gov't England 2016/10/05 Sci Rep. 2016 Oct 4; 6:34484. doi: 10.1038/srep34484" |
