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Infect Drug Resist

Title:		Rapid Identification of Carbapenemase-Producing Klebsiella pneumoniae Using Headspace Solid-Phase Microextraction Combined with Gas Chromatography-Mass Spectrometry
Author(s):		Luo H; Hang Y; Zhu H; Zhong Q; Peng S; Gu S; Fang X; Hu L;
Address:		"Jiangxi Provincial Key Laboratory of Medicine, Clinical Laboratory of the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People's Republic of China. Clinical Laboratory of Ganzhou People's Hospital, Ganzhou, Jiangxi, People's Republic of China"
Journal Title:		Infect Drug Resist
Year:		2023
Volume:		20230501
Issue:
Page Number:		2601 - 2609
DOI:		10.2147/IDR.S404742
ISSN/ISBN:		1178-6973 (Print) 1178-6973 (Electronic) 1178-6973 (Linking)
Abstract:		"BACKGROUND: Carbapenemase-producing Klebsiella pneumoniae is an unprecedented threat to public health, and its detection remains challenging. Analysis of microbial volatile organic compounds (VOCs) may offer a rapid way to determine bacterial antibiotic susceptibility. PURPOSE: The aim of this study was to explore the VOCs released by carbapenemase-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) using headspace solid-phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS). METHODS: Test bacteria were incubated in trypticase soy broth to the end of exponential growth phase, and imipenem was added in the middle time. Headspace VOCs were concentrated and analyzed using HS-SPME/GC-MS. RESULTS: The compound 3-methyl-1-butanol was found to be a biomarker among the 26 bacterial isolates (10 KPC-positive, 10 NDM-positive, 2 IMP-positive, 2 carbapenemase-negative CRKP, and 2 carbapenem-susceptible K. pneumonoiae). CONCLUSION: This study explored a promising new strategy for the screening of carbapenemase-producing CRKP strains. Further research with larger sample sizes will potentially accelerate the application of biomarkers in routine microbiology"
Keywords:		carbapenem-resistantKlebsiella pneumoniae headspace solid-phase microextraction combined with gas chromatography-mass spectrometry volatile organic compounds;
Notes:		"PubMed-not-MEDLINELuo, Hong Hang, Yaping Zhu, Hongying Zhong, Qiaoshi Peng, Suqin Gu, Shumin Fang, Xueyao Hu, Longhua eng New Zealand 2023/05/08 Infect Drug Resist. 2023 May 1; 16:2601-2609. doi: 10.2147/IDR.S404742. eCollection 2023"