| Title: | [Relationship between the Change Rules of Volatile Organic Compounds in Rat Muscle and Postmortem Interval] |
| Author(s): | Liu BB; Xia ZY; Ma JQ; Li P; Lu P; Zhou HM; |
| Address: | "School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, China" |
| DOI: | 10.3969/j.issn.1004-5619.2017.02.003 |
| ISSN/ISBN: | 1004-5619 (Print) 1004-5619 (Linking) |
| Abstract: | "OBJECTIVES: To explore the relationship between the change rules of volatile organic compounds ??OVOCs??d in rat muscle and postmortem interval ??OPMI??d. METHODS: A total of 120 healthy rats were divided randomly into 12 groups ??O10 for each group??d. After the rats were sacrificed by cervical dislocation, the bodies were kept at ??O25+/-1??d ?SG. Rat muscle samples were separately obtained at 12 PMI points, including 0, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 d. The VOCs in rat muscles were collected, detected and analyzed by headspace solid-phase microextraction ??OHS-SPME??d coupled to gas chromatography-mass spectrometer ??OGC-MS??d. RESULTS: In total, 15 species of VOCs were identified, including 9 aromatic compounds, 3 sulfur compounds, 2 aliphatic acids and 1 heterocyclic compound. The species of VOCs increased with PMI??_ no species were detected within 1 day, 3 species were detected on day 2, 9 on day 3, 11 on day 4, 14 from day 5 to 7, and 15 from day 8 to 10. Total peak area of 15 species of VOCs was significantly correlated to PMI ??Oadjusted R(2)=0.15-0.96??d??_ the regression function was y=-17.05 x(2)+ 164.36 x-246.36 ??Oadjusted R(2)=0.96??d from day 2 to 5, and y=2.24 x+101.13 ??Oadjusted R(2)=0.97??d from day 6 to 10. CONCLUSIONS: The change rules of VOCs in rat muscle are helpful for PMI estimation" |
| Keywords: | Animals Autopsy Gas Chromatography-Mass Spectrometry/*methods Muscles/*pathology Rats *Solid Phase Microextraction Volatile Organic Compounds/*chemistry volatile organic compounds forensic pathology gas chromatography-mass spectrometry muscles postmortem; |
| Notes: | "MedlineLiu, B B Xia, Z Y Ma, J Q Li, P Lu, P Zhou, H M chi China 2017/12/13 Fa Yi Xue Za Zhi. 2017 Apr; 33(2):120-124. doi: 10.3969/j.issn.1004-5619.2017.02.003. Epub 2017 Apr 25" |
