| Title: | Variation patterns in the content of glycosides during green tea manufacturing by a modification-specific metabolomics approach: Enzymatic reaction promoting an increase in the glycosidically bound volatiles at the pan firing stage |
| Author(s): | Li P; Zhu Y; Lu M; Yang C; Xie D; Tan J; Peng Q; Zhang Y; Ni D; Dai W; Lin Z; |
| Address: | "Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China; College of Horticulture and Forestry Science, Huazhong Agricultural University, 1 Shizishan Street, Hongshan District, Wuhan, Hubei 430070, People's Republic of China; Graduate School of Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, People's Republic of China. Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China. Agilent Technologies (China) Limited, 3 Wangjing North Road, Chaoyang District, Beijing 100102, People's Republic of China. College of Horticulture and Forestry Science, Huazhong Agricultural University, 1 Shizishan Street, Hongshan District, Wuhan, Hubei 430070, People's Republic of China. Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China. Electronic address: daiweidong@tricaas.com. Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China. Electronic address: linzhi@caas.cn" |
| DOI: | 10.1016/j.foodchem.2018.11.148 |
| ISSN/ISBN: | 1873-7072 (Electronic) 0308-8146 (Linking) |
| Abstract: | "The glycosides are presumed to influence the quality of green tea but the molecular mechanism behind remains unclear. To elucidate the contribution of glycosides to the flavor formation of green tea, changes of both glycosidically bound non-volatiles (GBNVs) and glycosidically bound volatiles (GBVs) during the manufacturing of green tea were investigated using a modification-specific metabolomics method. A total of 64 glycosides (47 GBNVs and 17 GBVs) were identified and their contents mainly changed during the pan firing and drying stages of green tea manufacturing. Notably, the contents of GBVs significantly increased by 1.12-4.46-fold during pan firing. Correlation analysis showed that the GBVs contents were negatively related to the contents of volatiles and glucose. Model experiments revealed that enzymatic synthesis contributed to the increase in the content of GBVs during the pan firing. This comprehensive study on the glycosides changes revealed the molecular bases for GBVs increments during the pan firing" |
| Keywords: | "Chromatography, High Pressure Liquid Enzymes/genetics/*metabolism Gas Chromatography-Mass Spectrometry/methods Glucose/analysis/isolation & purification Glycosides/analysis/chemistry/*metabolism Hot Temperature Metabolomics/*methods Solid Phase Microextra;" |
| Notes: | "MedlineLi, Pengliang Zhu, Yin Lu, Meiling Yang, Chen Xie, Dongchao Tan, Junfeng Peng, Qunhua Zhang, Yue Ni, Dejiang Dai, Weidong Lin, Zhi eng England 2019/01/07 Food Chem. 2019 May 1; 279:80-87. doi: 10.1016/j.foodchem.2018.11.148. Epub 2018 Dec 6" |
