| Title: | "Ten fatty acyl-CoA reductase family genes were essential for the survival of the destructive rice pest, Nilaparvata lugens" |
| Author(s): | Li DT; Dai YT; Chen X; Wang XQ; Li ZD; Moussian B; Zhang CX; |
| Address: | "State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Science, Zhejiang University, Hangzhou, China. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China. Universite Cote d'Azur, CNRS, Inserm, Institute of Biology Valrose (iBV), Parc Valrose, Nice, France. Institute of Insect Science, Zhejiang University, Hangzhou, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Fatty alcohols are the precursors of sex pheromone components, wax esters and hydrocarbons in insects. Fatty acyl-CoA reductases (FARs) are important enzymes required for the reduction of fatty alcohol and thereby contribute to the production of cuticular hydrocarbon (CHC). RESULTS: Based on bioinformatics analyses we identified 17 FAR genes in the brown planthopper, Nilaparvata lugens. RNA interference against these genes demonstrated that ten NlFAR genes were essential for the survival of N. lugens. For instance, knockdown of NlFAR5, 6, 11 or 15 was lethal and caused a slender body shape, while the old cuticles of the respective animals remained attached to the abdomen or failed to split open from the nota. Knockdown of NlFAR9 resulted in a phenotype, with a smooth body surface and a decrease in CHC amounts. Similarly, CHC deficiency in N. lugens resulted in increased adhesion of water droplets and secreted honeydew to the insect surface and the inability of N. lugens to survive in paddy fields with varying humidity. Knockdown of NlFAR1, 4, 5, 6, 8, 9, 11 and 13 additionally resulted in female adult infertility. CONCLUSION: The present study illustrates the structural and functional differences of FAR family genes and provides potential targets for RNA interference-based rice planthopper management. (c) 2020 Society of Chemical Industry" |
| Keywords: | Aldehyde Oxidoreductases Animals Female *Hemiptera RNA Interference Nilaparvata lugens cuticular hydrocarbon fatty acyl-CoA reductase lipid metabolism waterproofing;Animals; |
| Notes: | "MedlineLi, Dan-Ting Dai, Yi-Ting Chen, Xuan Wang, Xin-Qiu Li, Ze-Dong Moussian, Bernard Zhang, Chuan-Xi eng 31630057/National Natural Science Foundation of China/ 31871954/National Natural Science Foundation of China/ Research Foundation/ England 2020/01/30 Pest Manag Sci. 2020 Jul; 76(7):2304-2315. doi: 10.1002/ps.5765. Epub 2020 Feb 11" |
