| Title: | Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types |
| Address: | "Department of Neuroscience, Washington University School of Medicine in St. Louis, St. Louis, MO, USA. Department of Neuroscience, Washington University School of Medicine in St. Louis, St. Louis, MO, USA. holy@wustl.edu" |
| ISSN/ISBN: | 1095-9203 (Electronic) 0036-8075 (Print) 0036-8075 (Linking) |
| Abstract: | "Neural circuit analysis relies on having molecular markers for specific cell types. However, for a cell type identified only by its circuit function, the process of identifying markers remains laborious. We developed physiological optical tagging sequencing (PhOTseq), a technique for tagging and expression profiling of cells on the basis of their functional properties. PhOTseq was capable of selecting rare cell types and enriching them by nearly 100-fold. We applied PhOTseq to the challenge of mapping receptor-ligand pairings among pheromone-sensing neurons in mice. Together with in vivo ectopic expression of vomeronasal chemoreceptors, PhOTseq identified the complete combinatorial receptor code for a specific set of ligands" |
| Keywords: | "Animals Calcium/physiology Chemoreceptor Cells/*physiology Mice Mice, Transgenic Molecular Imaging/*methods Optical Imaging/*methods Pheromones/*physiology Protons Vomeronasal Organ;Neuroscience;" |
| Notes: | "MedlineLee, Donghoon Kume, Maiko Holy, Timothy E eng R01 DC005964/DC/NIDCD NIH HHS/ R01 DC010381/DC/NIDCD NIH HHS/ UF1 NS108176/NS/NINDS NIH HHS/ Research Support, N.I.H., Extramural 2019/12/14 Science. 2019 Dec 13; 366(6471):1384-1389. doi: 10.1126/science.aax8055" |
