| Title: | A prediction model using 2-propanol and 2-butanone in urine distinguishes breast cancer |
| Author(s): | Kure S; Satoi S; Kitayama T; Nagase Y; Nakano N; Yamada M; Uchiyama N; Miyashita S; Iida S; Takei H; Miyashita M; |
| Address: | "Department of Integrated Diagnostic Pathology, Nippon Medical School, 1-1-5, Sendagi, Bunkyoku, Tokyo, 113-8602, Japan. skure@nms.ac.jp. Department of Medicine, Mount Sinai Beth Israel, 281 First Avenue at 16th Street, New York, NY, 10003, USA. RIKEN KEIKI Co., Ltd., Minami-Sakaecho, Kasukabeshi, Saitama, 344-0057, Japan. Faculty of Medical Science, Nippon Sport Science University, 1221-1 Kamoshida-cho, Aoba-ku, Yokohama, Kanagawa, 227-0033, Japan. Koyukai Asakusa Clinic, 4-11-6, Asauksa, Taito-ku, Tokyo, 111-0032, Japan. Department of Cardiovascular Medicine, Tufts Medical Center, 800 Washington Street, Boston, MA, 02111, USA. Department of Breast Oncology, Nippon Medical School Chiba Hokusoh Hospital, 1715 Kamagari, Inzai, Chiba, 270-1694, Japan. Department of Breast Surgery and Oncology, Nippon Medical School Hospital, 1-1-5, Sendagi, Bunkyoku, Tokyo, 113-8603, Japan. Nippon Medical School, 1-1-5, Sendagi, Bunkyoku, Tokyo, 113-8602, Japan. Twin Peaks Laboratory of Medicine (TPLM), 34 Motai, Oaza, Obanazawa, Yamagata, 999-4331, Japan" |
| DOI: | 10.1038/s41598-021-99396-5 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Safe and noninvasive methods for breast cancer screening with improved accuracy are urgently needed. Volatile organic compounds (VOCs) in biological samples such as breath and blood have been investigated as noninvasive novel markers of cancer. We investigated volatile organic compounds in urine to assess their potential for the detection of breast cancer. One hundred and ten women with biopsy-proven breast cancer and 177 healthy volunteers were enrolled. The subjects were divided into two groups: a training set and an external validation set. Urine samples were collected and analyzed by gas chromatography and mass spectrometry. A predictive model was constructed by multivariate analysis, and the sensitivity and specificity of the model were confirmed using both a training set and an external set with reproducibility tests. The training set included 60 breast cancer patients (age 34-88 years, mean 60.3) and 60 healthy controls (age 34-81 years, mean 58.7). The external validation set included 50 breast cancer patients (age 35-85 years, mean 58.8) and 117 healthy controls (age 18-84 years, mean 51.2). One hundred and ninety-one compounds detected in at least 80% of the samples from the training set were used for further analysis. The predictive model that best-detected breast cancer at various clinical stages was constructed using a combination of two of the compounds, 2-propanol and 2-butanone. The sensitivity and specificity in the training set were 93.3% and 83.3%, respectively. Triplicated reproducibility tests were performed by randomly choosing ten samples from each group, and the results showed a matching rate of 100% for the breast cancer patient group and 90% for the healthy control group. Our prediction model using two VOCs is a useful complement to the current diagnostic tools. Further studies inclusive of benign tumors and non-breast malignancies are warranted" |
| Keywords: | "2-Propanol/*urine Adolescent Adult Aged Aged, 80 and over *Biomarkers, Tumor Breast Neoplasms/*diagnosis/*urine Butanones/*urine Case-Control Studies Female Gas Chromatography-Mass Spectrometry Humans Liquid Biopsy Middle Aged Neoplasm Grading Neoplasm St;" |
| Notes: | "MedlineKure, Shoko Satoi, Sera Kitayama, Toshihiko Nagase, Yuta Nakano, Nobuo Yamada, Marina Uchiyama, Noboru Miyashita, Satoshi Iida, Shinya Takei, Hiroyuki Miyashita, Masao eng England 2021/10/07 Sci Rep. 2021 Oct 5; 11(1):19801. doi: 10.1038/s41598-021-99396-5" |
