| Title: | Volatiles of the Apicomplexan Alga Chromera velia and Associated Bacteria |
| Author(s): | Koteska D; Marter P; Huang S; Pradella S; Petersen J; Schulz S; |
| Address: | "Institut fur Organische Chemie, Technische Universitat Braunschweig, Hagenring 30, 38106, Braunschweig, Germany. Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7B, 38124, Braunschweig, Germany" |
| ISSN/ISBN: | 1439-7633 (Electronic) 1439-4227 (Print) 1439-4227 (Linking) |
| Abstract: | "Volatiles released by the apicomplexan alga Chromera velia CCAP1602/1 and their associated bacteria have been investigated. A metagenome analysis allowed the identification of the most abundant heterotrophic bacteria of the phycosphere, but the isolation of additional strains showed that metagenomics underestimated the complexity of the algal microbiome, However, a culture-independent approach revealed the presence of a planctomycete that likely represents a novel bacterial family. We analysed algal and bacterial volatiles by open-system-stripping analysis (OSSA) on Tenax TA desorption tubes, followed by thermodesorption, cryofocusing and GC-MS-analysis. The analyses of the alga and the abundant bacterial strains Sphingopyxis litoris A01A-101, Algihabitans albus A01A-324, 'Coraliitalea coralii' A01A-333 and Litoreibacter sp. A01A-347 revealed sulfur- and nitrogen-containing compounds, ketones, alcohols, aldehydes, aromatic compounds, amides and one lactone, as well as the typical algal products, apocarotenoids. The compounds were identified by gas chromatographic retention indices, comparison of mass spectra and syntheses of reference compounds. A major algal metabolite was 3,4,4-trimethylcyclopent-2-en-1-one, an apocarotenoid indicating the presence of carotenoids related to capsanthin, not reported from algae so far. A low overlap in volatiles bouquets between C. velia and the bacteria was found, and the xenic algal culture almost exclusively released algal components" |
| Keywords: | *Alveolata/metabolism Gas Chromatography-Mass Spectrometry/methods Mass Spectrometry Alcohols/metabolism Bacteria Gc-ms apocarotenoids marine bacteria metagenomics volatile organic compounds; |
| Notes: | "MedlineKoteska, Diana Marter, Pia Huang, Sixing Pradella, Silke Petersen, Jorn Schulz, Stefan eng Research Support, Non-U.S. Gov't Germany 2022/11/24 Chembiochem. 2023 Feb 1; 24(3):e202200530. doi: 10.1002/cbic.202200530. Epub 2022 Dec 20" |
