| Title: | "Identification and Optimization of Microbial Attractants for Philornis downsi, an Invasive Fly Parasitic on Galapagos Birds" |
| Author(s): | Cha DH; Mieles AE; Lahuatte PF; Cahuana A; Lincango MP; Causton CE; Tebbich S; Cimadom A; Teale SA; |
| Address: | "College of Environmental Science and Forestry, State University of New York, Syracuse, NY, USA. USDA-ARS, US Pacific Basin Agricultural Research Laboratory, 64 Nowelo St., Hilo, HI, USA. Charles Darwin Foundation for the Galapagos Islands, Puerto Ayora, Santa Cruz Island, Galapagos Islands, Ecuador. Facultad de Ciencias Agricolas, Universidad Central del Ecuador, Quito, Ecuador. Department of Behavioural Biology, University of Vienna, 1090, Vienna, Austria. College of Environmental Science and Forestry, State University of New York, Syracuse, NY, USA. sateale@esf.edu" |
| DOI: | 10.1007/s10886-016-0780-1 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "We investigated the role of olfactory cues from actively fermenting yeast (Saccharomyces cerevisiae) in attraction of adult Philornis downsi and identified two synergistically attractive yeast volatiles. Larvae of this invasive fly parasitize the hatchlings of passerines and threaten the Galapagos avifauna. Gas chromatography coupled with electroantennographic detection (GC-EAD), coupled gas chromatography-mass spectrometry (GC-MS), and field trapping experiments were used to identify volatile compounds from a yeast-sugar solution. EAD responses were consistently elicited by 14 yeast volatiles. In a series of field trapping experiments, a mixture of the 14 EAD-active compounds was similarly attractive to P. downsi when compared to the yeast-sugar solution, and we found that acetic acid and ethanol were essential for attraction. A mixture of 0.03 % acetic acid and 3 % ethanol was as attractive as the 14-component blend, but was not as attractive as the yeast-sugar solution. Philornis downsi showed positive and negative dose-responses to acetic acid in the ranges of 0.01 ~ 0.3 % and 0.3 ~ 9 %, respectively. Further optimization showed that the mixture of 1 % acetic acid and 3 % ethanol was as attractive as the yeast-sugar solution. Both mixtures of acetic acid and ethanol were more selective than the yeast-sugar solution in terms of non-target moths and Polistes versicolor wasps captured. These results indicate that acetic acid and ethanol produced by yeasts are crucial for P. downsi attraction to fermented materials on which they feed as adults and can be used to manage this invasive fly in Galapagos" |
| Keywords: | "Animals Birds/*parasitology Dose-Response Relationship, Drug Fermentation Introduced Species Muscidae/drug effects/*microbiology/*physiology Olfactory Perception/drug effects Pheromones/chemistry/pharmacology Saccharomyces cerevisiae/*metabolism Volatiliz;" |
| Notes: | "MedlineCha, Dong H Mieles, Alejandro E Lahuatte, Paola F Cahuana, Andrea Lincango, Marie Piedad Causton, Charlotte E Tebbich, Sabine Cimadom, Arno Teale, Stephen A eng P 26556/FWF_/Austrian Science Fund FWF/Austria 2016/10/17 J Chem Ecol. 2016 Nov; 42(11):1101-1111. doi: 10.1007/s10886-016-0780-1. Epub 2016 Oct 15" |
