| Title: | A temperature-sensitive N-glycosylation mutant of S. cerevisiae that behaves like a cell-cycle mutant |
| Author(s): | Klebl F; Huffaker T; Tanner W; |
| DOI: | 10.1016/0014-4827(84)90573-1 |
| ISSN/ISBN: | 0014-4827 (Print) 0014-4827 (Linking) |
| Abstract: | "The temperature-sensitive S. cerevisiae mutant alg1-1, defective in the N-glycosylation of proteins, shows a first cycle arrest at the non-permissive temperature of 36 degrees C. The cell number increases by 50% and the absorbance approximately doubles. The budding index of 0.4 at 26 degrees C drops to 0.15 and DNA synthesis quickly comes to a halt at 36 degrees C. When the temperature is lowered again, budding and DNA synthesis start after a lag of 2-3 h; alpha-factor prevents both these processes in cells of mating type a. In addition, cells arrested at 26 degrees C in G1 with alpha-factor also do not start budding at the non-permissive temperature after removal of alpha-factor. The results support recent findings obtained with tunicamycin and suggest that at least one glycoprotein is required for G1-S phase transition in yeast" |
| Keywords: | "DNA, Fungal/biosynthesis Fungal Proteins/*physiology Glycoproteins/*physiology *Interphase Mating Factor Mutation Peptides/physiology RNA, Fungal/biosynthesis Saccharomyces cerevisiae/*cytology/genetics/metabolism Temperature;" |
| Notes: | "MedlineKlebl, F Huffaker, T Tanner, W eng GM 31318-21/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1984/02/01 Exp Cell Res. 1984 Feb; 150(2):309-13. doi: 10.1016/0014-4827(84)90573-1" |
