| Title: | Pitanga (Eugenia uniflora L.) fruit juice and two major constituents thereof exhibit anti-inflammatory properties in human gingival and oral gum epithelial cells |
| Author(s): | Josino Soares D; Walker J; Pignitter M; Walker JM; Imboeck JM; Ehrnhoefer-Ressler MM; Montenegro Brasil I; Somoza V; |
| Address: | "CAPES Foundation, Ministry of Education of Brazil, Brasilia - DF 70040-020, Brazil. Jessica.Walker@univie.ac.at" |
| ISSN/ISBN: | 2042-650X (Electronic) 2042-6496 (Linking) |
| Abstract: | "Pitanga, Eugenia uniflora L., is a tropical fruit, which may be consumed as juice. While beneficial health effects of Eugenia uniflora L. leaf extracts have extensively been studied, limited data are available on an anti-inflammatory potential of pitanga juice. The aim of the presented study was to investigate anti-inflammatory properties of pitanga juice with regards to a prevention of inflammation-related periodontal diseases. For this purpose, six healthy volunteers swirled pitanga juice, containing 35% pitanga pulp, for 10 min. Thereafter, oral gum epithelial cells were harvested using a sterile brush and stimulated with lipopolysaccharides from Porphyromonas gingivalis (PG-LPS) for 6 h. Furthermore, human gingival fibroblasts (HGF-1) were used to elucidate the anti-inflammatory potential of pitanga juice constituents, cyanidin-3-glucoside and oxidoselina-1,3,7(11)-trien-8-one, in juice representative concentrations of 119 mug ml(-1) and 30 mug ml(-1), respectively. For the first time, an anti-inflammatory impact of pitanga juice on gingival epithelial cells was shown by means of an attenuation of IL-8 release by 55 +/- 8.2% and 52 +/- 11% in non-stimulated and PG-LPS-stimulated cells, respectively. In addition, both cyanidin-3-glucoside and oxidoselina-1,3,7(11)-trien-8-one reduced the LPS-stimulated CXCL8 mRNA expression by 50 +/- 15% and 37 +/- 18% and IL-8 release by 52 +/- 9.9% and 45 +/- 3.7% in HGF-1 cells, when concomitantly incubated with 10 mug ml(-1)PG-LPS for 6 h, revealing an anti-inflammatory potential of the volatile compound oxidoselina-1,3,7(11)-trien-8-one for the first time" |
| Keywords: | Anthocyanins/pharmacology Anti-Inflammatory Agents/chemistry/*pharmacology Beverages/*analysis Cell Line Epithelial Cells/*drug effects Fibroblasts/drug effects Fruit/*chemistry Gingiva/*drug effects/metabolism Glucosides/pharmacology Humans Interleukin-8; |
| Notes: | "MedlineJosino Soares, Denise Walker, Jessica Pignitter, Marc Walker, Joel Michael Imboeck, Julia Maria Ehrnhoefer-Ressler, Miriam Margit Montenegro Brasil, Isabella Somoza, Veronika eng Research Support, Non-U.S. Gov't England 2014/09/18 Food Funct. 2014 Nov; 5(11):2981-8. doi: 10.1039/c4fo00509k" |
