| Title: | Breath and plasma metabolomics to assess inflammation in acute stroke |
| Author(s): | Ahmed W; White IR; Wilkinson M; Johnson CF; Rattray N; Kishore AK; Goodacre R; Smith CJ; Fowler SJ; |
| Address: | "Division of Infection, Immunity and Respiratory Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK. Manchester Institute of Biotechnology, University of Manchester, Manchester, UK. Laboratory for Environmental and Life Sciences, University of Nova Gorica, Nova Gorica, Slovenia. Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK. Greater Manchester Comprehensive Stroke Centre, Geoffrey Jefferson Brain Research Centre, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Salford, UK. Division of Cardiovascular Sciences, Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK. Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK. Greater Manchester Comprehensive Stroke Centre, Geoffrey Jefferson Brain Research Centre, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Salford, UK. Craig.Smith-2@manchester.ac.uk. Division of Cardiovascular Sciences, Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK. Craig.Smith-2@manchester.ac.uk. Division of Infection, Immunity and Respiratory Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK. Stephen.Fowler@manchester.ac.uk. NIHR Manchester Biomedical Research Centre, Manchester University Hospitals NHS Foundation Trust, Manchester, UK. Stephen.Fowler@manchester.ac.uk" |
| DOI: | 10.1038/s41598-021-01268-5 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Inflammation is strongly implicated in both injury and repair processes occurring after stroke. In this exploratory study we assessed the feasibility of repeated sampling of exhaled volatile organic compounds and performed an untargeted metabolomic analysis of plasma collected at multiple time periods after stroke. Metabolic profiles were compared with the time course of the inflammatory markers C-reactive protein (CRP) and interleukin-6 (IL-6). Serial breath sampling was well-tolerated by all patients and the measurement appears feasible in this group. We found that exhaled decanal tracks CRP and IL-6 levels post-stroke and correlates with several metabolic pathways associated with a post-stroke inflammatory response. This suggests that measurement of breath and blood metabolites could facilitate development of novel therapeutic and diagnostic strategies. Results are discussed in relation to the utility of breath analysis in stroke care, such as in monitoring recovery and complications including stroke associated infection" |
| Keywords: | "Aged Aged, 80 and over Biomarkers/blood/metabolism Breath Tests/*methods C-Reactive Protein/metabolism Exhalation Feasibility Studies Female Humans Inflammation/blood/complications/*metabolism Interleukin-6/blood Male Metabolomics Middle Aged Stroke/blood;" |
| Notes: | "MedlineAhmed, Waqar White, Iain R Wilkinson, Maxim Johnson, Craig F Rattray, Nicholas Kishore, Amit K Goodacre, Royston Smith, Craig J Fowler, Stephen J eng Research Support, Non-U.S. Gov't England 2021/11/11 Sci Rep. 2021 Nov 9; 11(1):21949. doi: 10.1038/s41598-021-01268-5" |
