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Eukaryot Cell

Title:		The volatome of Aspergillus fumigatus
Author(s):		Heddergott C; Calvo AM; Latge JP;
Address:		"Unite des Aspergillus, Institut Pasteur, Paris, France christoph.heddergott@pasteur.fr. Department of Biological Sciences, Northern Illinois University, Dekalb, Illinois, USA. Unite des Aspergillus, Institut Pasteur, Paris, France"
Journal Title:		Eukaryot Cell
Year:		2014
Volume:		20140606
Issue:		8
Page Number:		1014 - 1025
DOI:		10.1128/EC.00074-14
ISSN/ISBN:		1535-9786 (Electronic) 1535-9778 (Print) 1535-9786 (Linking)
Abstract:		"Early detection of invasive aspergillosis is absolutely required for efficient therapy of this fungal infection. The identification of fungal volatiles in patient breath can be an alternative for the detection of Aspergillus fumigatus that still remains problematic. In this work, we investigated the production of volatile organic compounds (VOCs) by A. fumigatus in vitro, and we show that volatile production depends on the nutritional environment. A. fumigatus produces a multiplicity of VOCs, predominantly terpenes and related compounds. The production of sesquiterpenoid compounds was found to be strongly induced by increased iron concentrations and certain drugs, i.e., pravastatin. Terpenes that were always detectable in large amounts were alpha-pinene, camphene, and limonene, as well as sesquiterpenes, identified as alpha-bergamotene and beta-trans-bergamotene. Other substance classes that were found to be present in the volatome, such as 1-octen-3-ol, 3-octanone, and pyrazines, were found only under specific growth conditions. Drugs that interfere with the terpene biosynthesis pathway influenced the composition of the fungal volatome, and most notably, a block of sesquiterpene biosynthesis by the bisphosphonate alendronate fundamentally changed the VOC composition. Using deletion mutants, we also show that a terpene cyclase and a putative kaurene synthase are essential for the synthesis of volatile terpenes by A. fumigatus. The present analysis of in vitro volatile production by A. fumigatus suggests that VOCs may be used in the diagnosis of infections caused by this fungus"
Keywords:		Alkyl and Aryl Transferases/genetics Aspergillus fumigatus/genetics/*metabolism Fungal Proteins/genetics Gene Knockout Techniques Metabolome Sesquiterpenes/*metabolism Volatile Organic Compounds/*metabolism;
Notes:		"MedlineHeddergott, C Calvo, A M Latge, J P eng Research Support, Non-U.S. Gov't 2014/06/08 Eukaryot Cell. 2014 Aug; 13(8):1014-25. doi: 10.1128/EC.00074-14. Epub 2014 Jun 6"