| Title: | Potent effect of KISS1-54 DNA vaccine compared with KISS1-10 DNA vaccine in inhibiting the fertility of female rats |
| Author(s): | Han Y; Peng X; Li K; Jiang X; Liu G; Yang L; Liang C; Zhao Y; Huang Y; E G; Zhao Y; Huang Y; |
| Address: | "Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing, PR China. Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, PR China. Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing, PR China. Electronic address: hyf65@163.com" |
| DOI: | 10.1016/j.vaccine.2018.09.053 |
| ISSN/ISBN: | 1873-2518 (Electronic) 0264-410X (Linking) |
| Abstract: | "BACKGROUND: Most studies on immunocastration currently focused on male animals. However, immunization of male animals does not completely inhibit sexual behavior and fertility. This study aimed to compare the immunocastration effect of KISS1 DNA vaccines encoding different lengths of kisspeptins in female rats for effective castration effects on both male and female rats. METHODS: Fifteen female rats were randomly divided into three groups. The rats in T1 group or T2 group was orally given respectively KISS1-54 or KISS1-10 DNA vaccines with fused tPA signal peptide, and the control group (Group C) was orally administered with empty vector vaccine, at a dose of 5?ª+x?ª+10(9) CFU/rat at weeks 0, 3 and 6 of the study. Blood samples were collected by retroorbital bleeding before primary immunization and at weeks 3 and 9 after primary immunization. RESULTS: Both KISS1-54 and KISS1-10 DNA vaccines induced the body's humoral immune response, and the anti-kisspeptin antibody titres in the T1 group were significantly higher than that in T2 and C groups (p?ª+ |
| Keywords: | "Animals Female Fertility/*drug effects Kisspeptins/*immunology RNA, Messenger/genetics/immunology Rats Vaccines, DNA/*pharmacology DNA vaccine Female rats Fertility Kisspeptin 10 Kisspeptin 54;" |
| Notes: | "MedlineHan, Yanguo Peng, Xiaoli Li, Kai Jiang, Xunping Liu, Guiqiong Yang, Liguo Liang, Caiyou Zhao, Yuhetian Huang, Yongjie E, Guangxin Zhao, Yongju Huang, Yongfu eng Research Support, Non-U.S. Gov't Netherlands 2018/10/03 Vaccine. 2018 Oct 29; 36(45):6631-6639. doi: 10.1016/j.vaccine.2018.09.053. Epub 2018 Sep 28" |
