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J Mass Spectrom

Title:		Qualitative screening for volatile organic compounds in human blood using solid-phase microextraction and gas chromatography-mass spectrometry
Author(s):		Gottzein AK; Musshoff F; Madea B;
Address:		"Institute of Legal Medicine, Toxicology, University of Bonn, Stiftsplatz 12, Bonn 53111, Germany. A.Gottzein@gmx.de"
Journal Title:		J Mass Spectrom
Year:		2010
Volume:		45
Issue:		4
Page Number:		391 - 397
DOI:		10.1002/jms.1723
ISSN/ISBN:		1096-9888 (Electronic) 1076-5174 (Linking)
Abstract:		"A fast and simple screening procedure using solid-phase microextraction and gas chromatography-mass spectrometry (SPME-GC-MS) in full-scan mode for the determination of volatile organic compounds (VOC) is presented. The development of a fast and simple screening technique for the simultaneous determination of various volatiles is of great importance, because of their widespread use, frequent occurrence in forensic toxicological questions and the fact that there is often no hint on involved substances at the crime scene. To simulate a screening procedure, eight VOC with different chemical characteristics were chosen (isoflurane, halothane, hexane, chloroform, benzene, isooctane, toluene and xylene). To achieve maximum sensitivity, variables that influence the SPME process, such as type of fiber, extraction and desorption temperature and time, agitation and additives were optimized by preliminary studies and by means of a central composite design. The limits of detection and recoveries ranged from 2.9 microg/l (xylene) to 37.1 microg/l (isoflurane) and 7.9% (chloroform) to 61.5% (benzene), respectively. This procedure can be used to answer various forensic and toxicological questions. The short time taken for the whole analytical procedure may make its eventual adoption for routine analysis attractive"
Keywords:		Analysis of Variance Dimethylpolysiloxanes/chemistry Forensic Medicine/methods Gas Chromatography-Mass Spectrometry/*methods Humans Hydrogen-Ion Concentration Nylons/chemistry Reproducibility of Results Salts/chemistry Sensitivity and Specificity Solid Ph;
Notes:		"MedlineGottzein, Anne Kathrin Musshoff, Frank Madea, Burkhard eng England 2010/03/20 J Mass Spectrom. 2010 Apr; 45(4):391-7. doi: 10.1002/jms.1723"