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FEBS Lett

Title:		Processing of Kex2 pro-region at two interchangeable cleavage sites
Author(s):		Germain D; Thomas DY; Boileau G;
Address:		"Departement de Biochimie, Universite de Montreal, Canada"
Journal Title:		FEBS Lett
Year:		1993
Volume:		323
Issue:		1-Feb
Page Number:		129 - 131
DOI:		10.1016/0014-5793(93)81463-a
ISSN/ISBN:		0014-5793 (Print) 0014-5793 (Linking)
Abstract:		"In Saccharomyces cerevisiae, the Kex2 endoprotease (Kex2p) is required for the proteolytic maturation of alpha-pheromone and also for the removal of its own pro-region. Kex2p is specific for pairs of basic amino acid residues. Two putative processing sites are present in the pro-region of Kex2p. We have expressed processing site mutants of Kex2p and assayed the production of active Kex2p. Mutations affecting either putative cleavage site do not alter the activity. However, mutations affecting both sites led to a reduction in both Kex2 activity and the amount of protein. These results suggest that removal of Kex2p pro-peptide is required for the production of a stable enzyme and can occur at either processing site"
Keywords:		"Enzyme Stability Immunoblotting *Proprotein Convertases Protein Precursors/metabolism *Protein Processing, Post-Translational Saccharomyces cerevisiae/*metabolism *Saccharomyces cerevisiae Proteins Subtilisins/*metabolism;"
Notes:		"MedlineGermain, D Thomas, D Y Boileau, G eng Research Support, Non-U.S. Gov't England 1993/05/24 FEBS Lett. 1993 May 24; 323(1-2):129-31. doi: 10.1016/0014-5793(93)81463-a"