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Fungal Genet Biol

Title:		"Smelling the difference: Transcriptome, proteome and volatilome changes after mating"
Author(s):		Freihorst D; Brunsch M; Wirth S; Krause K; Kniemeyer O; Linde J; Kunert M; Boland W; Kothe E;
Address:		"Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany. Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoll Institute, Adolf-Reichwein-Strasse 23, 07745 Jena, Germany. Max Planck Institute for Chemical Ecology, Department of Bioorganic Chemistry, Hans-Knoll-Strasse 8, 07745 Jena, Germany. Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany. Electronic address: erika.kothe@uni-jene.de"
Journal Title:		Fungal Genet Biol
Year:		2018
Volume:		20160901
Issue:
Page Number:		2 - 11
DOI:		10.1016/j.fgb.2016.08.007
ISSN/ISBN:		1096-0937 (Electronic) 1087-1845 (Linking)
Abstract:		"Mushrooms, such as Schizophyllum commune, have a specific odor. Whether this is linked to mating, prerequisite for mushroom formation, or also found in monokaryotic, unmated strains, was investigated with a comprehensive study on the transcriptome and proteome of this model organism. Mating interactions were investigated using a complete, cytosolic proteome map for unmated, monokaryotic, as well as for mated, dikaryotic mycelia. The regulations of the proteome were compared to transcriptional changes upon mating and to changes in smell by volatilome studies. We could show a good overlap between proteome and transcriptome data, but extensive posttranslational regulation was identified for more than 80% of transcripts. This suggests down-stream regulation upon interaction of mating partners and formation of the dikaryon that is competent to form fruiting bodies. The volatilome was shown to respond to mating by a broader spectrum of volatiles and increased emission of the mushroom smell molecules 3-octanone and 1-octen-3-ol, as well as ethanol and beta-bisabolol in the dikaryon. Putatively involved biosynthetic proteins like alcohol dehydrogenases, Ppo-like oxygenases, or sesquiterpene synthases showed correlating transcriptional regulation depending on either mono- or dikaryotic stages"
Keywords:		"*Gene Expression Profiling *Metabolomics Microbial Interactions Proteome/*analysis Recombination, Genetic Schizophyllum/genetics/*growth & development/*metabolism Volatile Organic Compounds/*metabolism Basidiomycete Mating Proteome Schizophyllum commune T;"
Notes:		"MedlineFreihorst, Daniela Brunsch, Melanie Wirth, Sophia Krause, Katrin Kniemeyer, Olaf Linde, Jorg Kunert, Maritta Boland, Wilhelm Kothe, Erika eng Research Support, Non-U.S. Gov't 2016/09/07 Fungal Genet Biol. 2018 Mar; 112:2-11. doi: 10.1016/j.fgb.2016.08.007. Epub 2016 Sep 1"