| Title: | Argentinean Andean propolis associated with the medicinal plant Larrea nitida Cav. (Zygophyllaceae). HPLC-MS and GC-MS characterization and antifungal activity |
| Author(s): | Aguero MB; Svetaz L; Sanchez M; Luna L; Lima B; Lopez ML; Zacchino S; Palermo J; Wunderlin D; Feresin GE; Tapia A; |
| Address: | "Instituto de Biotecnologia - Instituto de Ciencias Basicas, Universidad Nacional de San Juan, Av. Libertador General San Martin 1109 (O), 5400 San Juan, Argentina" |
| DOI: | 10.1016/j.fct.2011.05.008 |
| ISSN/ISBN: | 1873-6351 (Electronic) 0278-6915 (Linking) |
| Abstract: | "The chemical profile and botanical origin of Andean Argentinian propolis were studied by HPLC-ESI-MS/MS and GC-MS techniques as well as the antifungal activity according to CLSI protocols. Dermatophytes and yeasts tested were strongly inhibited by propolis extracts (MICs between 31.25 and 125 mug/mL). The main antifungal compounds were: 3'methyl-nordihydroguaiaretic acid (MNDGA) 1, nordihydroguaiaretic acid (NDGA) 2 and a NDGA derivative 3, showing strong activity against Trichophyton mentagrophytes, T. rubrum and Microsporum gypseum (MICs between 15.6 and 31.25 mug/mL). The lignans 1 and 2 showed activities against clinical isolates of Candidas spp., Cryptococcus spp., T. rubrum and T. mentagrophytes (MICs and MFCs between 31.25 and 62.5 mug/mL). The lignan and volatile organic compounds (VOCs) profiles from propolis matched with those of exudates of Larrea nitida providing strong evidences on its botanical origin. These results support that Argentinian Andean propolis are a valuable natural product with potential to improve human health. Six compounds (1-6) were isolated from propolis for the first time, while compounds 1 and 3-6 were reported for first time as constituents of L. nitida Cav" |
| Keywords: | "Antifungal Agents/*pharmacology Chromatography, High Pressure Liquid/*methods Gas Chromatography-Mass Spectrometry/*methods Larrea/*chemistry Magnetic Resonance Spectroscopy Mass Spectrometry/*methods Microbial Sensitivity Tests Propolis/*pharmacology;" |
| Notes: | "MedlineAguero, Maria Belen Svetaz, Laura Sanchez, Marianela Luna, Lorena Lima, Beatriz Lopez, Maria Liza Zacchino, Susana Palermo, Jorge Wunderlin, Daniel Feresin, Gabriela Egly Tapia, Alejandro eng Research Support, Non-U.S. Gov't England 2011/05/24 Food Chem Toxicol. 2011 Sep; 49(9):1970-8. doi: 10.1016/j.fct.2011.05.008. Epub 2011 May 12" |
