| Title: | Diversity of isoprene-degrading bacteria in phyllosphere and soil communities from a high isoprene-emitting environment: a Malaysian oil palm plantation |
| Author(s): | Carrion O; Gibson L; Elias DMO; McNamara NP; van Alen TA; Op den Camp HJM; Supramaniam CV; McGenity TJ; Murrell JC; |
| Address: | "School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. o.carrion-fonseca@uea.ac.uk. School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. UK Centre of Ecology & Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster, LA1 4AP, UK. Department of Microbiology, Faculty of Science, IWWR, Radboud University Nijmegen, Heyendaalseweg 135, NL-6525 AJ, Nijmegen, The Netherlands. School of Biosciences, Nottingham Centre of Sustainable Palm Oil, University of Nottingham-Malaysia, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia. School of Life Sciences, University of Essex, Colchester, UK. School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. j.c.murrell@uea.ac.uk" |
| DOI: | 10.1186/s40168-020-00860-7 |
| ISSN/ISBN: | 2049-2618 (Electronic) 2049-2618 (Linking) |
| Abstract: | "BACKGROUND: Isoprene is the most abundantly produced biogenic volatile organic compound (BVOC) on Earth, with annual global emissions almost equal to those of methane. Despite its importance in atmospheric chemistry and climate, little is known about the biological degradation of isoprene in the environment. The largest source of isoprene is terrestrial plants, and oil palms, the cultivation of which is expanding rapidly, are among the highest isoprene-producing trees. RESULTS: DNA stable isotope probing (DNA-SIP) to study the microbial isoprene-degrading community associated with oil palm trees revealed novel genera of isoprene-utilising bacteria including Novosphingobium, Pelomonas, Rhodoblastus, Sphingomonas and Zoogloea in both oil palm soils and on leaves. Amplicon sequencing of isoA genes, which encode the alpha-subunit of the isoprene monooxygenase (IsoMO), a key enzyme in isoprene metabolism, confirmed that oil palm trees harbour a novel diversity of isoA sequences. In addition, metagenome-assembled genomes (MAGs) were reconstructed from oil palm soil and leaf metagenomes and putative isoprene degradation genes were identified. Analysis of unenriched metagenomes showed that isoA-containing bacteria are more abundant in soils than in the oil palm phyllosphere. CONCLUSION: This study greatly expands the known diversity of bacteria that can metabolise isoprene and contributes to a better understanding of the biological degradation of this important but neglected climate-active gas. Video abstract" |
| Keywords: | Bacteria/classification/metabolism *Biodiversity Butadienes/metabolism *Hemiterpenes/metabolism Malaysia *Plant Leaves/microbiology *Soil *Soil Microbiology Climate DNA stable isotope probing Isoprene Isoprene monooxygenase Oil palm isoA; |
| Notes: | "MedlineCarrion, Ornella Gibson, Lisa Elias, Dafydd M O McNamara, Niall P van Alen, Theo A Op den Camp, Huub J M Supramaniam, Christina Vimala McGenity, Terry J Murrell, J Colin eng 694578-IsoMet/H2020 European Research Council/International Research Support, Non-U.S. Gov't England 2020/06/05 Microbiome. 2020 Jun 3; 8(1):81. doi: 10.1186/s40168-020-00860-7" |
