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RSC Adv

Title:		A targeted metabolomic protocol for quantitative analysis of volatile organic compounds in urine of children with celiac disease
Author(s):		Drabinska N; Azeem HA; Krupa-Kozak U;
Address:		"Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences Tuwima 10 Str. 10-748 Olsztyn Poland n.drabinska@pan.olsztyn.pl natalia_drabinska@wp.pl +48 89 524 01 24 +48 89 523 46 39. Centre for Analysis and Synthesis, Department of Chemistry, Lund University Naturvetarvagen 14/Solvegatan 39 A 221 00 Lund Sweden"
Journal Title:		RSC Adv
Year:		2018
Volume:		20181029
Issue:		64
Page Number:		36534 - 36541
DOI:		10.1039/c8ra07342b
ISSN/ISBN:		2046-2069 (Electronic) 2046-2069 (Linking)
Abstract:		"Volatile organic compounds (VOCs) in biological samples have gained popularity for disease monitoring and diagnosis. Celiac disease (CD) is one of the many prevalent health conditions which are challenging to diagnose. The aim of this study was to optimize a solid phase microextraction followed by gas chromatography-mass spectrometry, for quantitative analysis of a wide range of VOCs in the urine of patients with CD. Multivariate design of experiment was used to optimize the extraction conditions for the analysis of 15 urinary VOCs. Based on the performed experiments, extraction using 2.98 g of sodium chloride and 21 muL of 6 M hydrochloric acid for 15 min at 30 degrees C, using a CAR/PDMS fiber in headspace mode was found to be the most effective procedure for the analysis of the selected biomarkers. It was also demonstrated that the proposed method could distinguish between children with CD and healthy children based on the profile of VOCs. It is believed that quantitative analysis of these biomarkers will extend our understanding of CD and could be used for monitoring in patients under treatment"
Keywords:
Notes:		"PubMed-not-MEDLINEDrabinska, Natalia Azeem, Hafiz Abdul Krupa-Kozak, Urszula eng England 2018/10/29 RSC Adv. 2018 Oct 29; 8(64):36534-36541. doi: 10.1039/c8ra07342b. eCollection 2018 Oct 26"