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Front Physiol

Title:		An Orphan Pheromone Receptor Affects the Mating Behavior of Helicoverpa armigera
Author(s):		Cao S; Huang T; Shen J; Liu Y; Wang G;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, China Agricultural University, Beijing, China. Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China"
Journal Title:		Front Physiol
Year:		2020
Volume:		20200430
Issue:
Page Number:		413 -
DOI:		10.3389/fphys.2020.00413
ISSN/ISBN:		1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking)
Abstract:		"The Lepidoptera is the second largest insect order, which has the most extensive knowledge of sex pheromones and mechanisms of pheromone communication since the identification of the first insect pheromone in Bombyx mori. In the past 15 years, pheromone receptors have been identified and functionally characterized in many moth species. HarmOR14 is a typical pheromone receptor of Helicoverpa armigera which showed no response to the tested pheromones in Xenopus oocyte expression system, but its orthologous gene in Heliothis virescens, HvirOR14 could be activated by pheromones in the same expression system. To assess the possible functions of OR14 in vivo, in this study, we knocked out this gene using CRISPR/Cas9 system and compared the mating behaviors and EAG response to pheromones between the wild type and mutant strains. Our results showed that OR14 mutants did not affect the mating rate or the EAG response to pheromones but could prolong the mating duration and change the mating time in undefined manners, which extends our understanding to this kind of pheromone receptors"
Keywords:		CRISPR/Cas9 Eag Helicoverpa armigera mating behavior pheromone receptor;
Notes:		"PubMed-not-MEDLINECao, Song Huang, Tianyu Shen, Jie Liu, Yang Wang, Guirong eng Switzerland 2020/05/20 Front Physiol. 2020 Apr 30; 11:413. doi: 10.3389/fphys.2020.00413. eCollection 2020"