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Arch Microbiol

Title:		Production of volatile organic compounds (VOCs) by yeasts isolated from the ascocarps of black (Tuber melanosporum Vitt.) and white (Tuber magnatum Pico) truffles
Author(s):		Buzzini P; Gasparetti C; Turchetti B; Cramarossa MR; Vaughan-Martini A; Martini A; Pagnoni UM; Forti L;
Address:		"Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali, Sezione di Microbiologia Applicata, Universita di Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy. pbuzzini@unipg.it"
Journal Title:		Arch Microbiol
Year:		2005
Volume:		20051110
Issue:		3
Page Number:		187 - 193
DOI:		10.1007/s00203-005-0043-y
ISSN/ISBN:		0302-8933 (Print) 0302-8933 (Linking)
Abstract:		"Twenty-nine yeast strains were isolated from the ascocarps of black and white truffles (Tuber melanosporum Vitt. and Tuber magnatum Pico, respectively), and identified using a polyphasic approach. According to the conventional taxonomic methods, MSP-PCR fingerprinting and sequencing of the D1/D2 domain of 26S rDNA, the strains were identified as Candida saitoana, Debaryomyces hansenii, Cryptococcus sp., Rhodotorula mucilaginosa, and Trichosporon moniliiforme. All isolates assimilated L: -methionine as a sole nitrogen source and produced the volatile organic compounds (VOCs), 2-methyl butanol, 3-methyl butanol, methanethiol, S-methyl thioacetate, dimethyl sulfide, dimethyl disulfide, dimethyl trisulfide, dihydro-2-methyl-3(2H)-thiophenone and 3-(methylthio)-1-propanol (MTP). ANOVA analysis of data showed significant (P<0.01) differences in VOCs produced by different yeasts, with MTP as the major component (produced at concentrations ranging from 19.8 to 225.6 mg/l). In addition, since some molecules produced by the isolates of this study are also characteristic of truffle complex aroma, it is possible to hypothesize a complementary role of yeasts associated with this ecosystem in contributing to final Tuber spp. aroma through the independent synthesis of yeast-specific volatile constituents"
Keywords:		"*Ascomycota DNA/chemistry/genetics DNA Fingerprinting DNA, Fungal/analysis Gas Chromatography-Mass Spectrometry Methionine/metabolism Molecular Sequence Data Organic Chemicals/chemistry/*metabolism RNA, Ribosomal/genetics Sequence Analysis, DNA Yeasts/cla;"
Notes:		"MedlineBuzzini, Pietro Gasparetti, Chiara Turchetti, Benedetta Cramarossa, Maria Rita Vaughan-Martini, Ann Martini, Alessandro Pagnoni, Ugo Maria Forti, Luca eng Germany 2005/09/28 Arch Microbiol. 2005 Nov; 184(3):187-93. doi: 10.1007/s00203-005-0043-y. Epub 2005 Nov 10"