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Anal Biochem

Title:		Preparation of radiolabeled peptides via an iodine exchange reaction
Author(s):		Breslav M; McKinney A; Becker JM; Naider F;
Address:		"Department of Chemistry, City University of New York, Staten Island, New York, 10314, USA"
Journal Title:		Anal Biochem
Year:		1996
Volume:		239
Issue:		2
Page Number:		213 - 217
DOI:		10.1006/abio.1996.0317
ISSN/ISBN:		0003-2697 (Print) 0003-2697 (Linking)
Abstract:		"Peptides labeled with radioactive 125I can be detected at the extremely low concentrations necessary for receptor binding studies and medical applications. Traditional methods of iodination often lead to inactive peptides due to excessive iodination, nonspecific iodination, or oxidative damage to the peptide. In this work these disadvantages are circumvented by labeling 127I containing peptides of a predetermined biological activity using a radioactive 125I exchange reaction with Na125I. Specific radioactivity up to 9.8 Ci/mmol was reached in a simple and efficient procedure"
Keywords:		"Chromatography, High Pressure Liquid Diiodotyrosine/chemistry *Iodine Radioisotopes Mating Factor Peptides/*chemistry Pheromones/chemistry Radioligand Assay/*methods Saccharomyces cerevisiae;"
Notes:		"MedlineBreslav, M McKinney, A Becker, J M Naider, F eng GM22086/GM/NIGMS NIH HHS/ GM22087/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1996/08/01 Anal Biochem. 1996 Aug 1; 239(2):213-7. doi: 10.1006/abio.1996.0317"