| Title: | Gastropods and Insects Prefer Different Solanum dulcamara Chemotypes |
| Author(s): | Calf OW; Huber H; Peters JL; Weinhold A; Poeschl Y; van Dam NM; |
| Address: | "Molecular Interaction Ecology, Institute for Water and Wetland Research (IWWR), Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, the Netherlands. o.calf@science.ru.nl. Experimental Plant Ecology, Institute for Water and Wetland Research (IWWR), Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, the Netherlands. Molecular Plant Physiology, Institute for Water and Wetland Research (IWWR), Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, the Netherlands. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103, Leipzig, Germany. Institute of Computer Science, Martin Luther University Halle-Wittenberg, Von-Seckendorff-Platz 1, 06120, Halle, Germany. Molecular Interaction Ecology, Institute for Water and Wetland Research (IWWR), Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, the Netherlands. nicole.vandam@idiv.de. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103, Leipzig, Germany. nicole.vandam@idiv.de. Institute of Biodiversity, Friedrich Schiller University Jena, Dornburger-Str. 159, 07743, Jena, Germany. nicole.vandam@idiv.de" |
| DOI: | 10.1007/s10886-018-0979-4 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking) |
| Abstract: | "Solanum dulcamara (Bittersweet nightshade) shows significant intraspecific variation in glycoalkaloid (GA) composition and concentration. We previously showed that constitutive differences in overall GA levels are correlated with feeding preference of the grey field slug (GFS; Deroceras reticulatum). One particularly preferred accession, ZD11, contained low GA levels, but high levels of previously unknown structurally related uronic acid conjugated compounds (UACs). Here we test whether different slug species as well as insect herbivores show similar feeding preferences among six S. dulcamara accessions with different GA chemotypes. In addition, we investigate whether slug feeding can lead to induced changes in the chemical composition and affect later arriving herbivores. A leaf disc assay using greenhouse-grown plants showed that three slug species similarly preferred accessions with low GA levels. Untargeted metabolomic analyses showed that previous slug feeding consistently increased the levels of N-caffeoyl-putrescine and a structurally related metabolite, but not the levels of GAs and UACs. Slug-induced responses only affected slug preference in one accession. A common garden experiment using the same six accessions revealed that ZD11 received the highest natural gastropod feeding damage, but suffered the lowest damage by specialist flea beetles. The latter preferred to feed on accessions with high GA levels. Our study indicates that different selection pressures imposed by generalist gastropods and specialist insects may explain part of the observed chemical diversity in S. dulcamara" |
| Keywords: | "Alkaloids/chemistry/metabolism/pharmacology Animals Chromatography, High Pressure Liquid Gastropoda/drug effects/*physiology Herbivory/drug effects Insecta/drug effects/*physiology Metabolome Plant Leaves/chemistry/metabolism Principal Component Analysis;" |
| Notes: | "MedlineCalf, Onno W Huber, Heidrun Peters, Janny L Weinhold, Alexander Poeschl, Yvonne van Dam, Nicole M eng 823.01.007/Nederlandse Organisatie voor Wetenschappelijk Onderzoek/ 2018/07/03 J Chem Ecol. 2019 Feb; 45(2):146-161. doi: 10.1007/s10886-018-0979-4. Epub 2018 Jul 2" |
