| Title: | Persistence of a Yeast-Based (Hanseniaspora uvarum) Attract-and-Kill Formulation against Drosophila suzukii on Grape Leaves |
| Author(s): | Bianchi F; Spitaler U; Castellan I; Cossu CS; Brigadoi T; Dumenil C; Angeli S; Robatscher P; Vogel RF; Schmidt S; Eisenstecken D; |
| Address: | "Laboratory for Flavours and Metabolites, Institute for Agricultural Chemistry and Food Quality, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, Italy. Chair of Technical Microbiology, TUM School of Life Sciences, Technical University of Munich, Gregor-Mendel-Strasse 4, 85354 Freising, Germany. Entomology Group, Institute for Plant Health, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, Italy. Department of Crop Sciences, Institute of Plant Protection, University of Natural Resources and Life Sciences, Gregor-Mendel-Strasse 33, 1180 Vienna, Austria. Faculty of Science and Technology, Free University of Bozen-Bolzano, 39100 Bolzano, Italy" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "The production of phagostimulant and attractive volatile organic compounds (VOCs) by yeasts can be exploited to improve the efficacy of attract-and-kill formulations against the spotted wing drosophila (SWD). This study evaluated the persistence over one week of a yeast-based formulation under greenhouse conditions. Potted grape plants were treated with: (i) potato dextrose broth (PDB), (ii) PDB containing spinosad (PDB + S), and (iii) H. uvarum fermentation broth grown on PDB containing spinosad (H. u. + S). Laboratory trials were performed to determine the survival and the oviposition rate of SWD after exposure to treated leaves. Ion-exchange chromatography was performed to measure carbohydrates, sugar alcohols, and organic acids on leaf surfaces, while amino acids were assessed through liquid chromatography-mass-spectrometry. Additionally, the VOCs released by plants treated with H.uvarum were collected via closed-loop-stripping analysis and compared to those emitted by untreated leaves. A higher mortality was observed for adult SWDs in contact with H. uvarum containing spinosad compared to PDB containing spinosad. Generally, a decrease in the amounts of non-volatile compounds was observed over time, though numerous nutrients were still present one week after treatment. The application of the yeast-based formulation induced the emission of VOCs by the treated leaves. The concentration of 2-phenylethanol, one of the main VOCs emitted by yeasts, decreased over time. These findings describe the presence of potential phagostimulants and compounds attractive to SWD in a yeast-based attract-and-kill formulation and demonstrate the efficacy of the formulation over one week" |
| Keywords: | VOCs metabolites pest control spinosad spotted wing drosophila; |
| Notes: | "PubMed-not-MEDLINEBianchi, Flavia Spitaler, Urban Castellan, Irene Cossu, Carlo S Brigadoi, Timothy Dumenil, Claire Angeli, Sergio Robatscher, Peter Vogel, Rudi F Schmidt, Silvia Eisenstecken, Daniela eng Switzerland 2020/11/22 Insects. 2020 Nov 18; 11(11):810. doi: 10.3390/insects11110810" |
