| Title: | The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway |
| Author(s): | Bhattacharyya RP; Remenyi A; Good MC; Bashor CJ; Falick AM; Lim WA; |
| Address: | "Department of Cellular and Molecular Pharmacology, University of California-San Francisco, 600 16th Street, San Francisco, CA 94143-2240, USA" |
| ISSN/ISBN: | 1095-9203 (Electronic) 0036-8075 (Linking) |
| Abstract: | "Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties" |
| Keywords: | "Adaptor Proteins, Signal Transducing/*chemistry/genetics/*metabolism Allosteric Regulation Amino Acid Motifs Binding Sites Crystallography, X-Ray Down-Regulation Enzyme Activation *MAP Kinase Signaling System Mitogen-Activated Protein Kinases/*chemistry/*;" |
| Notes: | "MedlineBhattacharyya, Roby P Remenyi, Attila Good, Matthew C Bashor, Caleb J Falick, Arnold M Lim, Wendell A eng Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2006/01/21 Science. 2006 Feb 10; 311(5762):822-6. doi: 10.1126/science.1120941. Epub 2006 Jan 19" |
