| Title: | "Strain of Fusarium oxysporum isolated from almond hulls produces styrene and 7-methyl-1,3,5-cyclooctatriene as the principal volatile components" |
| Author(s): | Beck JJ; Merrill GB; Palumbo JD; O'Keeffe TL; |
| Address: | "Western Regional Research Center, U.S. Department of Agriculture, Albany, California 94710, USA. john.beck@ars.usda.gov" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "An isolated strain of Fusarium oxysporum from the hulls of Prunus dulcis (sweet almond) was found to produce relatively large quantities of the hydrocarbons styrene and two isomers of 7-methyl-1,3,5- cyclooctatriene (MCOT). Production of styrene and MCOT was reproduced on a small scale using potato dextrose agar as a growth medium and scaled up using 1 L of inoculated potato dextrose broth. The compounds were trapped as volatile organic compounds (VOCs) onto solid-phase microextraction (SPME) for small scale and Tenax for large scale and then isolated using standard high-performance liquid chromatography (HPLC) methods. Styrene was authenticated by a comparison to the retention times, fragmentation patterns, and calculated retention indices of a commercially available sample. The identity of MCOT was verified by a short chemical synthesis and a comparison of spectroscopic data to the isolated sample. A biosynthetic scheme of styrene is proposed on the basis of a (13)C-labeling study. This is the first report of MCOT isolated as a natural product" |
| Keywords: | Cyclooctanes/analysis/*metabolism Fusarium/chemistry/isolation & purification/*metabolism Prunus/*microbiology Styrene/analysis/*metabolism Volatile Organic Compounds/analysis/*metabolism; |
| Notes: | "MedlineBeck, John J Merrill, Glory B Palumbo, Jeffrey D O'Keeffe, Teresa L eng Research Support, U.S. Gov't, Non-P.H.S. 2008/11/13 J Agric Food Chem. 2008 Dec 10; 56(23):11392-8. doi: 10.1021/jf802570w" |
