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Curr Genet

Title:		"The pleiotropic vegetative and sexual development phenotypes of Neurospora crassa arise from double mutants of the calcium signaling genes plc-1, splA2, and cpe-1"
Author(s):		Barman A; Tamuli R;
Address:		"Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781 039, India. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781 039, India. ranjantamuli@iitg.ernet.in"
Journal Title:		Curr Genet
Year:		2017
Volume:		20170306
Issue:		5
Page Number:		861 - 875
DOI:		10.1007/s00294-017-0682-y
ISSN/ISBN:		1432-0983 (Electronic) 0172-8083 (Linking)
Abstract:		"We investigated phenotypes of the double mutants of the calcium (Ca(2+)) signaling genes plc-1, splA2, and cpe-1 encoding for a phospholipase C1 (PLC-1), a secretory phospholipase A(2) (sPLA(2)), and a Ca(2+)/H(+) exchanger (CPE-1), respectively, to understand the cell functions regulated by their genetic interactions. Mutants lacking plc-1 and either splA2 or cpe-1 exhibited numerous defects including reduced colonial growth, stunted aerial hyphae, premature conidiation on plates with delayed germination, inappropriate conidiation in submerged culture, and lesser mycelial pigmentation. Moreover, the ?Osplc-1; ?OssplA2 and ?Osplc-1; ?Oscpe-1 double mutants were female-sterile when crossed with wild type as the male parent. In addition, ?Osplc-1, ?OssplA2, and ?Oscpe-1 single mutants displayed higher carotenoid accumulation and an increased level of intracellular reactive oxygen species (ROS). Therefore, the pleiotropic phenotype of the double mutants of plc-1, splA2, and cpe-1 suggested that the genetic interaction of these genes plays a critical role for normal vegetative and sexual development in N. crassa"
Keywords:		"Antioxidants/metabolism Biomass *Calcium Signaling Fungal Proteins/*genetics/*metabolism Gene Expression Regulation, Fungal *Hyphae *Mutation Neurospora crassa/*physiology *Phenotype Pheromones/metabolism Reactive Oxygen Species/metabolism Ca2+ signaling;"
Notes:		"MedlineBarman, Ananya Tamuli, Ranjan eng BT/PR3635/BCE/8/892/2012/Department of Biotechnology, Ministry of Science and Technology/ 2017/03/08 Curr Genet. 2017 Oct; 63(5):861-875. doi: 10.1007/s00294-017-0682-y. Epub 2017 Mar 6"