| Title: | Validation of biofilm formation on human skin wound models and demonstration of clinically translatable bacteria-specific volatile signatures |
| Author(s): | Ashrafi M; Novak-Frazer L; Bates M; Baguneid M; Alonso-Rasgado T; Xia G; Rautemaa-Richardson R; Bayat A; |
| Address: | "Plastic & Reconstructive Surgery Research, Division of Musculoskeletal & Dermatological Sciences, School of Biological Sciences, University of Manchester, Manchester, UK. Manchester University NHS Foundation Trust, Wythenshawe Hospital, Manchester, UK. Bioengineering Group, School of Materials, University of Manchester, Manchester, UK. Manchester Academic Health Science Centre, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester and Manchester University NHS Foundation Trust, Manchester, UK. MCBA Consulting, Cardiff, Wales, UK. Division of Infection, Immunity and Respiratory Medicine, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK. Plastic & Reconstructive Surgery Research, Division of Musculoskeletal & Dermatological Sciences, School of Biological Sciences, University of Manchester, Manchester, UK. ardeshir.bayat@manchester.ac.uk. Manchester University NHS Foundation Trust, Wythenshawe Hospital, Manchester, UK. ardeshir.bayat@manchester.ac.uk. Bioengineering Group, School of Materials, University of Manchester, Manchester, UK. ardeshir.bayat@manchester.ac.uk" |
| DOI: | 10.1038/s41598-018-27504-z |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | Biofilms are major contributors to delayed wound healing and there is a need for clinically relevant experimental models to assess theranostics. Microorganisms release volatile organic compounds (VOCs) and the ability to identify these in infected cutaneous wounds could lead to efficient non-invasive diagnosis. The aims here were to develop and assess bacterial biofilm formation and identify their VOC profiles in an in vitro model and validate in human ex vivo incisional and excisional cutaneous wound models. Biofilm development was assessed using multiple microscopy techniques with biofilm-forming deficient controls and quantified using metabolic and biomass assays; and VOC production measured by gas chromatography-mass spectrometry. The production of most VOCs was affected by biofilm development and model used. Some VOCs were specific either for planktonic or biofilm growth. The relative abundance of some VOCs was significantly increased or decreased by biofilm growth phase (P < 0.05). Some Staphylococcus aureus and Pseudomonas aeruginosa VOCs correlated with biofilm metabolic activity and biomass (R /=0.5). We present for the first time bacterial biofilm formation in human ex vivo cutaneous wound models and their specific VOC profiles. These models provide a vehicle for human skin-relevant biofilm studies and VOC detection has potential clinical translatability in efficient non-invasive diagnosis of wound infection |
| Keywords: | Adult Bacteriological Techniques/*methods *Biofilms Female Humans Male Middle Aged Pseudomonas aeruginosa/metabolism/pathogenicity/physiology Skin/*microbiology Staphylococcal Infections/*microbiology Staphylococcus aureus/metabolism/pathogenicity/physiol; |
| Notes: | "MedlineAshrafi, Mohammed Novak-Frazer, Lilyann Bates, Matthew Baguneid, Mohamed Alonso-Rasgado, Teresa Xia, Guoqing Rautemaa-Richardson, Riina Bayat, Ardeshir eng Wellcome Trust/United Kingdom BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't Validation Study England 2018/06/23 Sci Rep. 2018 Jun 21; 8(1):9431. doi: 10.1038/s41598-018-27504-z" |
