| Title: | Skin ethanol gas measurement system with a biochemical gas sensor and gas concentrator toward monitoring of blood volatile compounds |
| Author(s): | Arakawa T; Aota T; Iitani K; Toma K; Iwasaki Y; Mitsubayashi K; |
| Address: | "Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: arakawa.bdi@tmd.ac.jp. Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan; Postdoctoral Research Fellow PD, Japan Society for the Promotion of Science, 5-3-1 Kojimatchi, Chiyoda-ku, Tokyo 102-0083, Japan. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan. Faculty of Chemistry, Materials and Bioengineering, Kansai University, Osaka, Japan. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan; Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: m.bdi@tmd.ac.jp" |
| DOI: | 10.1016/j.talanta.2020.121187 |
| ISSN/ISBN: | 1873-3573 (Electronic) 0039-9140 (Linking) |
| Abstract: | "We developed a biochemical gas sensor (bio-sniffer) using the enzymatic reaction of alcohol dehydrogenase (ADH) to target ethanol in skin gas. By introducing a gas concentrator using liquid nitrogen, we constructed a highly sensitive system for skin gas measurements. The ethanol bio-sniffer was built from an optical-fiber probe employing an ADH enzyme membrane, an UV-LED light source for excitation, and a photomultiplier tube. Ethanol was measured by detecting the autofluorescence of the coenzyme NADH due to the enzymatic reaction of ADH. We established a system for measuring concentrated gases by connecting the sensor with a gas concentrator and introducing concentrated skin gas to the sensing surface. This suppressed diffusion of the concentrated gases to achieve maximum fluorescence intensity by optimizing the measurement system. The calibration curve from obtained peak values showed ethanol gas can be measured over 1-3100 ppb, which included skin gas concentrations during alcohol consumption. Finally, when applied to measurements of ethanol in skin gas following alcohol consumption, the output was found to be dependent on concentration, similarly to using standard gases. Consecutive measurements were possible using periodic sampling with 6-min intervals for 180 min of monitoring. Skin ethanol concentrations rose from 20 min after consuming the alcohol, exhibited a peak value of 25 ppb skin gas ethanol at around 60 min, and gradually declined. Thus, the system can be used for non-invasive percutaneous evaluation of human volatile organic chemicals in blood" |
| Keywords: | *Biosensing Techniques Breath Tests Ethanol Gases Humans *Volatile Organic Compounds Biosensor Enzyme Gas concentrator Gas sensor Skin gas ppb gas sensing; |
| Notes: | "MedlineArakawa, Takahiro Aota, Takashi Iitani, Kenta Toma, Koji Iwasaki, Yasuhiko Mitsubayashi, Kohji eng Netherlands 2020/09/06 Talanta. 2020 Nov 1; 219:121187. doi: 10.1016/j.talanta.2020.121187. Epub 2020 May 30" |
